您好，欢迎访问新疆农业科学院 机构知识库！
新疆农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Over-expression of GhUGP1 in upland cotton improves fibre quality and reduces fibre sugar content

文献类型： 外文期刊

作者： Li, Bo 1 ; Yang, Yang 1 ; Hu, Wen-Ran 1 ; Li, Xiao-Dong 1 ; Cao, Jia-Qiang 1 ; Fan, Ling 1 ;

作者机构： 1.Xinjiang Acad Agr Sci, Inst Nucl & Biol Technol, Urumqi 830091, Peoples R China

关键词： agronomic traits;cellulose;fibre quality;gene expression;sugar;transgenic line;uridine diphosphate glucose pyrophosphorylase

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： GhUGP1 (cotton uridine diphosphate glucose pyrophosphorylase) is one of the most highly expressed genes and proteins in fast elongation cotton fibres. The objective of this study was to determine whether fibre quality and reduced sugar content could be improved in fibres by over-expressing GhUGP1 in cotton plants under conditions of low autumn temperatures and large diurnal temperature differences. A plant GhUGP1 vector was constructed and introduced into the 3-6-7 cotton line using agrobacterium-mediated transformation. Following selection with kanamycin sulphate and polymerase chain reaction (PCR) and Southern blotting analyses, three independent homozygous transgenic lines were selected for molecular and chemical study. Compared with wild type, the transcriptional activity of GhUGP1 was higher and more long lasting in the transgenic lines. Fibres were longer and stronger, soluble and reduced sugar levels were significantly decreased, and cellulose levels were significantly increased. In addition, plant height also increased by 40.1-45.1%. These results demonstrate that GhUGP1 expression was considerably increased in the transgenic plants, accelerating the conversion of oligosaccharides to polysaccharides in the developing cotton fibre.

  • 相关文献

[1]Molecular Cloning, Structural Characterization, and Ripening-Related Expression of Lipoxygenase Genes from Three Persimmon Cultivars Differing in Postharvest Ripening Rate. Hou, Yali,Ban, Qiuyan,Zhang, Ting,Rao, Jingping,Huber, Donald J.,Dong, Xiaoqing,Chang, Xiaoxiao,Zhang, Ting.

[2]Identification of ten mevalonate enzyme-encoding genes and their expression in response to juvenile hormone levels in Leptinotarsa decemlineata (Say). Li, Qian,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[3]Natural variation reveals that OsSAP16 controls low-temperature germination in rice. Wang, Xiang,Zou, Baohong,Shao, Qiaolin,Cui, Yongmei,Lu, Shan,Zhang, Yan,Huang, Ji,Hua, Jian,Huang, Quansheng,Hua, Jian. 2018

[4]Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in Rice. Zhu, Chun Q.,Zhang, Jun H.,Zhu, Lian F.,Zhong, Chu,Bai, Zhi G.,Sajid, Hussain,Cao, Xiao C.,Jin, Qian Y.,Sun, Li M.,Abliz, Buhailiqem,Hu, Wen J.. 2018

[5]Impact of postharvest nitric oxide treatment on antioxidant enzymes and related genes in banana fruit in response to chilling tolerance. Wu, Bin,Li, Xueping,Chen, Weixin,Guo, Qin,Li, Qingpeng,Ha, Yiming,Wu, Bin. 2014

[6]Sucrose and citric acid accumulations in melon genotypes with different sugar and acid contents. Tang, Mi,Zhang, Bao-cai,Xie, Jun-jun,Bie, Zhi-long,Wu, Ming-zhu,Yi, Hong-ping,Feng, Jong-xin,Tang, Mi. 2012

[7]Improved approaches of in situ hybridization and in situ enzyme histochemistry for gene expression analyses in plants. Liu, QF,Hao, ZR,Shen, DL. 1998

[8]Molecular and Biochemical Evidence for Phenylpropanoid Synthesis and Presence of Wall-linked Phenolics in Cotton Fibers. Fan, Ling,Hu, Wen-Ran,Hao, Xiao-Yan,Wang, Dong-Mei,Yuan, Hui,Yan, Hong-Ying,Shi, Wei-Jun. 2009

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台