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Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)

文献类型： 外文期刊

作者： Wang, Guanying 1 ; Xu, Zhongping 1 ; Wang, Fuqiu 1 ; Huang, Yuefan 1 ; Xin, Yanfeng 1 ; Liang, Sijia 1 ; Li, Bo 1 ; Si, Huan 1 ; Sun, Lin 1 ; Wang, Qiongqiong 1 ; Ding, Xiao 1 ; Zhu, Xiangqian 1 ; Chen, Luo 1 ; Yu, Lu 1 ; Lindsey, Keith 3 ; Zhang, Xianlong 1 ; Jin, Shuangxia 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Nucl & Biol Technol, Xinjiang Key Lab Crop Biotechnol, Urumqi 830000, Xinjaing, Peoples R China

3.Univ Durham, Dept Biosci, Durham DH1 3LE, England

关键词： Cotton; CRISPR; nCas9; dCas9; dCpf1; Adenine base editors (ABEs); Off-target mutations

期刊名称：BMC BIOLOGY （ 影响因子：7.364； 五年影响因子：8.641 ）

ISSN：

年卷期： 2022 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Background Base editors (BEs) display diverse applications in a variety of plant species such as Arabidopsis, rice, wheat, maize, soybean, and cotton, where they have been used to mediate precise base pair conversions without the collateral generation of undesirable double-stranded breaks (DSB). Studies of single-nucleotide polymorphisms (SNPs) underpinning plant traits are still challenging, particularly in polyploidy species where such SNPs are present in multiple copies, and simultaneous modification of all alleles would be required for functional analysis. Allotetraploid cotton has a number of homoeologous gene pairs located in the A and D sub-genomes with considerable SNPs, and it is desirable to develop adenine base editors (ABEs) for efficient and precise A-to-G single-base editing without DSB in such complex genome. Results We established various ABE vectors based on different engineered adenosine deaminase (TadA) proteins fused to Cas9 variants (dCas9, nCas9), enabling efficient A to G editing up to 64% efficiency on-target sites of the allotetraploid cotton genome. Comprehensive analysis showed that GhABE7.10n exhibited the highest editing efficiency, with the main editing sites specifically located at the position A5 (counting the PAM as positions 21-23). Furthermore, DNA and RNA off-target analysis of cotton plants edited with GhABE7.10n and GhABE7.10d by whole genome and whole-transcriptome sequencing revealed no DNA off-target mutations, while very low-level RNA off-target mutations were detected. A new base editor, namely GhABE7.10dCpf1 (7.10TadA + dCpf1), that recognizes a T-rich PAM, was developed for the first time. Targeted A-to-G substitutions generated a single amino acid change in the cotton phosphatidyl ethanolamine-binding protein (GhPEBP), leading to a compact cotton plant architecture, an ideotype for mechanized harvesting of modern cotton production. Conclusions Our data illustrate the robustness of adenine base editing in plant species with complex genomes, which provides efficient and precise toolkit for cotton functional genomics and precise molecular breeding.

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