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Protective Effects of Blueberry Anthocyanins against H2O2-Induced Oxidative Injuries in Human Retinal Pigment Epithelial Cells

文献类型: 外文期刊

作者: Huang, Wu-Yang 1 ; Wu, Han 1 ; Li, Da-Jing 1 ; Song, Jiang-Feng 1 ; Xiao, Ya-Dong 1 ; Liu, Chun-Quan 1 ; Zhou, Jian-Zh 1 ;

作者机构: 1.Jiangsu Acad Agr Sci, Inst Farm Prod Proc, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China

3.Shanghai Jiao Tong Univ, Sch Agr & Biol, Bor S Luh Food Safety Res Ctr, Key Lab Urban Agr South,Dept Food Sci & Engn, Shanghai 200240, Peoples R China

关键词: age-related macular degeneration;antioxidant;blueberry anthocyanins;oxidative stress;retinal pigment epithelial cells

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )

ISSN: 0021-8561

年卷期: 2018 年 66 卷 7 期

页码:

收录情况: SCI

摘要: Blueberry anthocyanins are considered protective of eye health because of their recognized antioxidant properties. In this study, blueberry anthocyanin extract (BAE), malvidin (Mv), malvidin-3-glucoside (Mv-3-glc), and malvidin-3-galactoside (Mv-3-gal) all reduced H2O2-induced oxidative stress by decreasing the levels of reactive oxygen species and malondialdehyde and increasing the levels of superoxide dismutase, catalase, and glutathione peroxidase in human retinal pigment epithelial cells. BAE and the anthocyanin standards enhanced cell viability from 63.69 +/- 3.36 to 86.57 +/- 6.92% (BAE), 115.72 +/- 23.41% (Mv), 98.15 +/- 9.39% (Mv-3-glc), and 127.97 +/- 20.09% (Mv-3-gal) and significantly inhibited cell apoptosis (P < 0.01 for all). Mitogen-activated-protein-kinase pathways, including ERK1/2 and p38, were involved in the bioactivities. In addition, the anthocyanins decreased vascular-endothelial-cell-growth-factor levels and activated Akt-signal pathways. These combined results supported the hypothesis that blueberry anthocyanins could inhibit the induction and progression of age-related macular degeneration (AMD) through antioxidant mechanisms.

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