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A simple method for obtaining rice black-streaked dwarf virus-infected small brown planthopper nymphs

文献类型： 外文期刊

作者： Zhou, Changwei 1 ; Li, Shuo 2 ; Zhou, Yijun 2 ; Fan, Yongjian 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

关键词： RBSDV;SBPH spawning on infected plants;Viruliferous nymphs

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2018 年 251 卷

页码：

收录情况： SCI

摘要： Rice black-streaked dwarf virus (RBSDV), an important rice virus, is transmitted by vector small brown planthopper (SBPH) in a persistent manner, but not transovarial transmission. In order to obtain viruliferous SBPH nymphs for relevant research, a simple and reliable method was developed, through allowing SBPH adults laying eggs on RBSDV-infected rice plants. The results showed the hatching nymphs on diseased plants could early acquire virus, and the virus was detected in 2nd-instar nymphs from the spawning method, which was earlier than insect feed on diseased plant. The average viruliferous rate of SBPH from the spawning method was 32.9%, which was not lower than the feeding diseased plant method. The novel method was very easy to operate and time-saving, facilitating the study on the interaction between RBSDV and SBPH nymphs (especially young 2nd 4th instar nymphs), such as, the effect of RBSDV on nymph development, host plant orientation preference of viruliferous nymph, identification of viral interacting protein in nymph, etc.

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[1]Identification of virus-derived siRNAs and their targets in RBSDV-infected rice by deep sequencing. Li, Yanwu,Sun, Feng,Du, Linlin,Xu, Qiufang,Zhou, Tong,Zhou, Yijun,Fan, Yongjian,Li, Yanwu,E, Zhiguo. 2018

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