文献类型： 外文期刊
作者： Wang, Yun 1 ; Wang, Qian 1 ; Wu, Ai-hua 2 ; Hao, Zhen-ping 1 ; Liu, Xian-jin 2 ;
作者机构： 1.Jinling Inst Technol, Coll Hort, Nanjing 210038, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Key Lab Food Qual & Safety Jiangsu Prov,Minist Ag, State Key Lab Breeding Base,Inst Food Qual Safety, Key Lab Control Technol & Stand Agroprod Safety &, Nanjing 210014, Jiangsu, Peoples R China
关键词： CrylAb;Phage display;BBMV-Peptide sandwich-ELISA
期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）
ISSN： 0003-2697
年卷期： 2017 年 539 卷
页码：
收录情况： SCI
摘要： Traditional ELISA methods of using animal immunity yield antibodies for detection Cry toxin. Not only is this incredibly harmful to the animals, but is also time-intensive. Here we developed a simple method to yield the recognition element. Using a critical selection strategy and immunoassay we confirmed a clone from the Ph.D-C7C phage library, which has displayed the most interesting Cry1Ab-binding characteristics examined in this study (Fig. 1). The current study indicates that isolating peptide is an alternative method for the preparation of a recognition element, and that the developed assay is a potentially useful tool for detecting Cry1Ab. (C) 2017 Published by Elsevier Inc.
- 相关文献
作者其他论文 更多>>
-
Effects of neuropeptide F signaling on feeding, growth and development of Plutella xylostella (L.) larvae
作者：Shen, Cheng;Wu, Jiayi;Huang, Ziyan;He, Meng;Chen, Wei;Ilyas, Naila;Zhang, Xiao;Chen, Chengyu;Xu, Chongxin;Xie, Yajing;Liu, Yuan;Liu, Xianjin;Zhu, Qing;Shen, Cheng;Huang, Ziyan;Liu, Yuan;Liu, Xianjin;Wu, Jiayi;He, Meng;Xu, Chongxin;Xie, Yajing;Liu, Yuan;Liu, Xianjin;Zhu, Qing;Wang, Yun
关键词：Plutella xylostella; Neuropeptide F signaling; Feeding regulation
-
Target Ligand Separation and Identification of Isoforsythiaside as a Histone Lysine-Specific Demethylase 1 Covalent Inhibitor Against Breast Cancer Metastasis
作者：Gu, Mengzhen;Xu, Xiaoqing;Wang, Xiaoping;Wang, Yun;Zhao, Yu;Hu, Xiaoxian;Han, Chao;Zhu, Lu;Deng, Zhenzhong
关键词：
-
Optimizing planting density enhances the multi-scale structural characteristics and in vitro digestibility of maize starch via modulating the size distribution of granules
作者：Wu, Xiaorong;Zhai, Mingming;Wang, Zihao;Han, Qingfang;Guo, Ke;Liang, Wenxin;Wang, Qian;Herburger, Klaus;Li, Jingyuan;Zhong, Yuyue
关键词：Gricultural modification; Starch structure; Functional properties
-
Metabolic profilings of rat INS-1 beta-cells under changing levels of essential amino acids
作者：Xu, Lianbin;Lin, Xueyan;Hu, Zhiyong;Hou, Qiuling;Wang, Yun;Wang, Zhonghua;Li, Xiuli
关键词：
-
Integration of transcriptomics and metabolomics provides metabolic and functional insights into reduced insulin secretion in MIN6 beta-cells exposed to deficient and excessive arginine
作者：Xu, Lianbin;Lin, Xueyan;Hu, Zhiyong;Hou, Qiuling;Wang, Yun;Wang, Zhonghua;Li, Xiuli
关键词：arginine availability; functional integrity; glucose-stimulated insulin secretion; insulin release; metabolomics; MIN6 beta-cell; transcriptomics
-
Cloning and expression of Lactobacillus brevis beta-glucosidase and its effect on the aroma of strawberry wine
作者：Li, Xiaonan;Xia, Xiudong;Wang, Yun;Xu, Zhuang;Zhou, Jianzhong;Li, Xiaonan;Xia, Xiudong;Wang, Zhe;Dai, Yiqiang;Yin, Liqing;Xu, Zhuang;Zhou, Jianzhong;Wang, Zhe;Dai, Yiqiang;Yin, Liqing;Zhou, Jianzhong
关键词：
-
Physicochemical characteristics of ginger essential oil nanoemulsion encapsulated by zein/NaCas and antimicrobial control on chilled chicken
作者：Tang, Minmin;Liu, Fang;Wang, Daoying;Sun, Zhilan;Xu, Weimin;Tang, Minmin;Liu, Fang;Wang, Qian;Wang, Debao;Wang, Daoying;Zhu, Yongzhi;Sun, Zhilan;Xu, Weimin;Wang, Qian
关键词：Essential oil; Pseudomonas aeruginosa; Staphylococcus aureus; Antibacterial activity; Low-energy emulsification method
