文献类型： 外文期刊
作者： Wang, Yun 1 ; Wang, Qian 1 ; Wu, Ai-hua 2 ; Hao, Zhen-ping 1 ; Liu, Xian-jin 2 ;
作者机构： 1.Jinling Inst Technol, Coll Hort, Nanjing 210038, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Key Lab Food Qual & Safety Jiangsu Prov,Minist Ag, State Key Lab Breeding Base,Inst Food Qual Safety, Key Lab Control Technol & Stand Agroprod Safety &, Nanjing 210014, Jiangsu, Peoples R China
关键词： CrylAb;Phage display;BBMV-Peptide sandwich-ELISA
期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）
ISSN： 0003-2697
年卷期： 2017 年 539 卷
页码：
收录情况： SCI
摘要： Traditional ELISA methods of using animal immunity yield antibodies for detection Cry toxin. Not only is this incredibly harmful to the animals, but is also time-intensive. Here we developed a simple method to yield the recognition element. Using a critical selection strategy and immunoassay we confirmed a clone from the Ph.D-C7C phage library, which has displayed the most interesting Cry1Ab-binding characteristics examined in this study (Fig. 1). The current study indicates that isolating peptide is an alternative method for the preparation of a recognition element, and that the developed assay is a potentially useful tool for detecting Cry1Ab. (C) 2017 Published by Elsevier Inc.
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