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Effects of cyclophosphamide on antioxidative and immune functions of Nile tilapia (Oreochromis Niloticus) via the TLR-NF-Kappa B signaling pathway

文献类型： 外文期刊

作者： Cao, Li-ping 1 ; Du, Jin-liang 1 ; Jia, Rui 1 ; Ding, Wei-dong 2 ; Xu, Pao 1 ; Yin, Guo-jun 1 ; Zhang, Ting 3 ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr & Rural Affairs, Wuxi 214081, Jiangsu, Peoples R China

2.Chinese Acad Fishery Sci, Int Joint Res Lab Fish immunopharmacol, Wuxi 2140814, Jiangsu, Peoples R China

3.Nanjing Med Univ, Affiliated Wuxi Matem & Child Hlth Care Hosp, Wuxi 214002, Jiangsu, Peoples R China

关键词： Cyclophosphamide; Oreochromis niloticus; Immunosuppression; TLR-NF-kappa B signaling pathway

期刊名称：AQUATIC TOXICOLOGY （ 影响因子：4.964； 五年影响因子：5.071 ）

ISSN： 0166-445X

年卷期： 2021 年 239 卷

页码：

收录情况： SCI

摘要： Intensive aquaculture often results in immunosuppression in fish, which may cause a series of diseases. In this study, to investigate the immunosuppressive mechanisms in fish, tilapia were intrapleural injected cyclophosphamide (CTX) at the doses of 10, 25, 50, 75 and 100 mg.kg(-1) to induce immunosuppression. We determined the viability of immune cells, the content of lysozyme (LZM) and immunoglobulin M (IgM), the levels of nitric oxide (NO) and antioxidant parameters. Meanwhile, the mRNA levels of complement C3 (c3), igm and the genes associated with the TLR-NF-kappa B signaling pathway in the head kidney (HK) and spleen were also determined. The results showed that CTX had a significant cytotoxic effect on peripheral blood leukocytes, HK macrophages and spleen cells in a dose-dependent manner. The protein and mRNA levels of C3 and IgM were down-regulated with the increase of CTX concentrations in serum, HK and/or spleen. The NO and LZM contents decreased significantly in HK and spleen after CTX treatments with 75 and 100 mg.kg(-1). CTX treatments with 50, 75 and/or 100 mg.kg(-1) markedly decreased the antioxidant ability and enhanced lipid peroxidation in HK and spleen. Furthermore, qPCR data showed that CTX treatments with 50-100 mg.kg(-1) clearly down-regulated the mRNA levels of tlr2, myd88, irak1, traf6, nf kappa b1, nf kappa b2, il-6, il-10 and tnf-alpha in the HK and/or spleen. Overall results suggested that CTX treatment had a cytotoxic effect on immune cells, induced lipid peroxidation, decreased the antioxidant capacity and inhibited immune function. The immunosuppressive mechanisms of CTX may be associated with the TLR-NF-kappa B signaling pathway.

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