文献类型: 外文期刊
作者: Jia, Fengjing 1 ; Wang, Jiayi 1 ; Peng, Jinxiu 1 ; Zhao, Ping 1 ; Kong, Ziqing 2 ; Wang, Kairong 1 ; Yan, Wenjin 1 ; Wang 1 ;
作者机构: 1.Lanzhou Univ, Inst Biochem & Mol Biol, Sch Life Sci, Key Lab Preclin Study New Drugs Gansu Prov,Sch Ba, Lanzhou 730000, Gansu, Peoples R China
2.Jiangsu Acad Agr
关键词: antimicrobial peptide;polybia-CP;stability;D-amino acid substitution
期刊名称:ACTA BIOCHIMICA ET BIOPHYSICA SINICA ( 影响因子:3.848; 五年影响因子:3.67 )
ISSN: 1672-9145
年卷期: 2017 年 49 卷 10 期
页码:
收录情况: SCI
摘要: With the increasing emergence of resistant microbes toward conventional antimicrobial agents, there is an urgent need for the development of antimicrobial agents with novel action mode. Antimicrobial peptides (AMPs) are believed to be one kind of ideal alternatives. However, AMPs can be easily degraded by protease, which limited their therapeutic use. In the present study, D-amino acid substitution strategy was employed to enhance the stability of polybia-CP. We investigated the stability of peptides against the degradation of trypsin and chymotrypsin by determining the antimicrobial activity or determining the HPLC profile of peptides after incubation with proteases. Our results showed that both the all D-amino acid derivative (D-CP) and partial D-lysine substitution derivative (D-lys-CP) have an improved stability against trypsin and chymotrypsin. Although D-CP takes left-hand alpha-helical conformation and D-lys-CP loses some alpha-helical content, both of the D-amino acid-substituted derivatives maintain their parental peptides' membrane active action mode. In addition, D-lys-CP showed a slight weaker antimicrobial activity than polybia-CP, but the hemolytic activity decreased greatly. These results suggest that D-CP and D-lys-CP can offer strategy to improve the property of AMPs and may be leading compounds for the development of novel antimicrobial agents.
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