A non-enzymatic electrochemical biosensor based on SiO2-Au nanoparticles for hemoglobin detection
文献类型： 外文期刊
作者： Li, Caihong 1 ; Li, Jian 3 ; Tang, Haowei 4 ; Yang, Xiaodi 1 ; Fei, Qiqi 1 ; Sun, Chong 2 ;
作者机构： 1.Nanjing Normal Univ, Coll Chem & Mat Sci, Jiangsu Key Lab New Power Batteries, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Nanjing 210023, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Peoples R China
3.Nanjing Univ Tradit Chinese Med, Affiliated Hosp, Dept Neurosurg, Nanjing 210029, Jiangsu, Peoples R China
4.Nanjing Foreign Language Sch, Nanjing 210008, Peoples R China
期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）
ISSN： 1759-9660
年卷期： 2017 年 9 卷 8 期
页码：
收录情况： SCI
摘要： In this study, a simple non-enzymatic hemoglobin (Hb) biosensor based on SiO2-Au nanoparticles (SiO2-Au NPs) was prepared and investigated. The structure and morphology of the SiO2-Au NPs were characterized by transmission electron microscopy (TEM) and UV-vis spectroscopy. Fourier transform infrared spectra (FTIR) and circular dichroism (CD) spectra were obtained to test the biocompatibility of the SiO2-Au NPs. The electrochemical properties were determined via cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). Under the optimized conditions, the linear regression equation between the peak currents and the concentration of Hb was i (mu A) = -51.57269 + 83.14106c (mg mL(-1)) (R-2 = 0.9979) with a detection limit of 204.46 ng mL (1) . In addition, the electrochemical biosensor possessed good anti-interference performance. Due to the superior performance of SiO2-Au NPs, this method shows great potential applications for the direct sensing of Hb for the purpose of evaluating meat quality.
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