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UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot

文献类型： 外文期刊

作者： Wu, Xinxin 1 ; Gong, Qinghua 3 ; Ni, Xiaopeng 1 ; Zhou, Yong 1 ; Gao, Zhihong 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Lab Fruit Tree Biotechnol, Nanjing, Jiangsu, Peoples R China

2.Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing, Jiangsu, Peoples R China

3.Adm Bur Sun Yat Sens Mausoleum, Nanjing, Jiangsu, Peoples R China

关键词： anthocyanins content;enzyme activity;Japanese apricot;RNA-seq;transcriptome analysis;UFGT;variegation;nonsynonymous mutation

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Japanese apricot (Prunus mume Sieb. et Zucc.) is an important ornamental plant in China. One of the traits of petals color variegation is attractive, but its formation mechanism is unclear. In our study, RNA-seq technology was employed to characterize the transcriptome response to the mutation of "Fuban Tiaozhi" associated with petals variegation in Japanese apricot. As a result, 4,579,040 (white-flowered, WF) and 7,269,883 (red-flowered, RF) reads were mapped to P. persica genes, while 5,006,676 (WF) and 7,907,436 (RF) were mapped to P. persica genomes. There were 960 differentially expressed genes (DEGs) identified. Gene ontology analysis showed that these genes involved in 37 functional groups including 19 biological processes, 10 cellular components and eight molecular functions. Pathway enrichment annotation demonstrated that highly ranked genes were associated with flavonoid biosynthesis, anthocyanin biosynthesis, anthocyanins transports, plant hormone signal transduction, and transcriptional factors. The expression patterns part of them were validated by qRT-PCR. We found that UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) gene showed differential expression pattern. The UFGT enzyme activities in RF had a significantly higher than that of WF and lower in the initial stage and increased when the red appeared in the petals, which is identical to the accumulation of anthocyanins. And we also validated the SNPs, leading to the nonsynonymous mutations, in the UFGT by Sanger sequencing which may affect the enzyme activity. In summary, our results providemolecular candidates for better understanding themechanisms of the variegation in Japanese Apricot.

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