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Antibody Developments and Immunoassays for Organophosphorus Chemicals: A Review

文献类型： 外文期刊

作者： Hua, Xiude 1 ; Eremin, Sergei A. 3 ; Liu, Fengquan 1 ; Wang, Minghua 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, State & Local Joint Engn Res Ctr Green Pesticide, Nanjing 210095, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing, Jiangsu, Peoples R China

3.Russian Acad Sci, Fed Res Ctr Fundamentals Biotechnol, AN Bach Inst Biochem, Moscow 119071, Russia

4.Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119991, Russia

5.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

关键词： Organophosphorus;immunoassay;hapten;antibody;conjugate;pesticides

期刊名称：CURRENT ORGANIC CHEMISTRY （ 影响因子：2.18； 五年影响因子：2.173 ）

ISSN： 1385-2728

年卷期： 2017 年 21 卷 26 期

页码：

收录情况： SCI

摘要： Organophosphorus (OP) chemicals are mainly used as pesticides to control pests in both agricultural and domestic settings. The widespread and frequent use of OP pesticides has resulted in their presence as residues in environment and food. Due to their high toxicity and powerful inhibitors of cholinesterases, it is essential to monitor the residual OP chemicals in the environment and organism. Immunoassays for OP chemicals are now well-established techniques, and they meet the demand for rapid, simple, cost-effective on-site analysis. Haptens, antibodies, and labels are the key components of immunoassays for OP chemicals. This review gives an overview of the design and synthesis of immunizing and coating haptens, coupling of haptens to a carrier, reported antibodies against OP chemicals (e.g., polyclonal, monoclonal and recombinant antibody), and traditional and novel labeling materials for immunoassay signal detection (e.g., radioactive isotope, enzyme, fluorescent compound, and colloidal gold). In addition, we discussed future considerations and opportunities for advancing the development of immunoassays for OP chemicals.

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