Biodegradation of 3-methyldiphenylether (MDE) by Hydrogenophaga atypical strain QY7-2 and cloning of the methy-oxidation gene mdeABCD
文献类型： 外文期刊
作者： Yang, Qian 1 ; Cai, Shu 2 ; Dong, Shaowei 1 ; Chen, Lulu 1 ; Chen, Jifei 1 ; Cai, Tianming 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2016 年 6 卷
页码：
收录情况： SCI
摘要： 3-Methyldiphenylether (MDE) is an important alkyl-substituted diphenyl ether compound that is widely used as an intermediate in the synthesis of pyrethroid insecticides. An efficient MDE-degrading strain QY7-2, identified as Hydrogenophaga atypical, was isolated from activated sludge for the first time. Strain QY7-2 can utilize MDE as the sole carbon and energy source and completely mineralize MDE. The degradation pathway of MDE was proposed in the strain through metabolites identification. A gene cluster involving in methy-oxidation of MDE was cloned from QY7-2 and expressed in Escherichia coli BL21 (DE3), and the products were purified by SDS-PAGE. The specific activities of the recombinant enzymes MdeAB, MdeC and MdeD were 113.8 +/- 3.5, 274.5 +/- 6.2 and 673.4 +/- 8.7 nmol min(-1) mg(-1), respectively. These results provide the biochemical and genetic foundation of microbial degradation pathway of MDE and benefit the bioremediation of MDE-contaminated environments.
- 相关文献
作者其他论文 更多>>
-
Resistance patterns and molecular basis to ACCase-inhibiting herbicides
作者：Yang, Qian;Wei, Tian;Zhu, Jinlei;Lv, Min;Deng, Wei;Liu, Longwei;Yang, Xia;Li, Yongfeng
关键词：ACCase gene mutation; cross-resistance; metamifop; target site-based resistance
-
Removal of aqueous Cr(VI) by green synthesized sulfide iron nanoparticles loaded corn straw biochar: Performance, mechanism, and DFT calculations
作者：Rong, Kun;Li, Xueping;Yang, Qian;Zhang, Zaiwang;Li, Ruimin;Zhao, Lingzi;Zheng, Huanqiang;Rong, Kun;Liu, Zhaohui;Yao, Qiang
关键词：Sulfide modification; Green synthesis; Biochar; DFT; Aqueous Cr(VI)
-
CALB1: A novel antiviral factor in chicken ileal mucus
作者：Lu, Danqing;Wu, Yang;Lin, Jian;Fu, Mei;Wu, Yaotang;Lin, Jian;Yang, Qian;Wang, Zhisheng;Lin, Jian
关键词：Avian ileum mucus; CALB1; Antiviral activity; Binding calcium
-
Ile-1781-Leu Target Mutation and Non-Target-Site Mechanism Confer Resistance to Acetyl-CoA Carboxylase-Inhibiting Herbicides in Digitaria ciliaris var. chrysoblephara
作者：Yang, Qian;Zhu, Jinlei;Wei, Tian;Lv, Min;Yang, Xia;Li, Yongfeng
关键词：Digitaria ciliaris var; chrysoblephara; metamifop; cyhalofop-butyl; ACCase gene mutation; multiple gene copies; non-target-site resistance; weed control
-
Metabolic Resistance to Acetyl-CoA Carboxylase-Inhibiting Herbicide Cyhalofop-Butyl in a Chinese Echinochloa crus-galli Population
作者：Yang, Qian;Zhu, Jinlei;Wei, Tian;Lv, Min;Yang, Xia;Li, Yongfeng
关键词：resistance mechanisms; metabolism; cyhalofop-butyl; Echinochloa crus-galli; resistance pattern; multiple-resistance
-
From nasal to basal: single-cell sequencing of the bursa of Fabricius highlights the IBDV infection mechanism in chickens
作者：Shah, Abid Ullah;Zuo, Jinjiao;Lin, Jian;Shah, Abid Ullah;Li, Yuchen;Yu, Qinghua;Yang, Qian;Ouyang, Wei;Wang, Zhisheng;Shi, Song
关键词：Nasal cavity; Infectious bursal disease virus; Bursa of Fabricius; Single cell RNA-sequence; RNA interference
-
Enhanced metabolism confers a high level of cyhalofop-butyl resistance in a Chinese sprangletop (Leptochloa chinensis (L.) Nees) population
作者：Deng, Wei;Yang, Mengting;Li, Yang;Xia, Zhiming;Chen, Yongrui;Yuan, Shuzhong;Yang, Qian
关键词：cyhalofop‐ butyl; Leptochloa chinensis; herbicide resistance; non‐ target‐ site resistance (NTSR); enhanced metabolism
