文献类型： 外文期刊
作者： Wang, Ruping 1 ; Xu, Huiyong 1 ; Du, Liangcheng 2 ; Chou, Shan-Ho 3 ; Liu, Hongxia 1 ; Liu, Youzhou 5 ; Liu, Fengquan; 1 ;
作者机构： 1.Nanjing Agr Univ, Key Lab Integrated Management Crop Dis & Pests, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
2.Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA
3.Natl Chung Hsing Univ, Inst Biochem, Taichung 40227, Taiwan
4.Natl Chung Hsing Univ, NCHU Agr Biotechnol Ctr, Taichung 40227, Taiwan
5.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2016 年 6 卷
页码：
收录情况： SCI
摘要： Lysobacter species are Gram-negative bacteria that are emerging as new sources of antibiotics, including HSAF (Heat Stable Antifungal Factor), which was identified from L. enzymogenes with a new mode of action. LesR, a LuxR solo, was recently shown to regulate the HSAF biosynthesis via an unidentified mechanism in L. enzymogenes OH11. Here, we used a comparative proteomic approach to identify the LesR targets and found that LesR influenced the expression of 33 proteins belonging to 10 functional groups, with 9 proteins belonging to the TBDR (TonB-Dependent Receptor) family. The fundamental role of bacterial TBDR in nutrient uptake motivates us to explore their potential regulation on HSAF biosynthesis which is also modulated by nutrient condition. Six out of 9 TBDR coding genes were individually in-frame deleted. Phenotypic and gene-expression assays showed that TBDR7, whose level was lower in a strain overexpressing lesR, was involved in regulating HSAF yield. TBDR7 was not involved in the growth, but played a vital role in transcribing the key HSAF biosynthetic gene. Taken together, the current lesR-based proteomic study provides the first report that TBDR7 plays a key role in regulating antibiotic (HSAF) biosynthesis, a function which has never been found for TBDRs in bacteria.
- 相关文献
作者其他论文 更多>>
-
First Report of Bacterial Fruit Blotch Caused by Kosakonia cowanii on Trichosanthis fructus in Jiangsu Province, China
作者：Chen, Xian;Tang, Bao;Zhao, Yancun;Liu, Fengquan;Chen, Xian;Tang, Bao;Laborda, Pedro
关键词：bacterial fruit blotch; Kosakonia cowanii; Trichosanthis fructus
-
Unveiling the Role of Diffusible Signal Factor-Family Quorum Sensing Signals in Regulating Behavior of Xanthomonas and Lysobacter
作者：Li, Kaihuai;Ma, Chaoyun;Xiong, Chunlan;Zhou, Xue;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Ma, Chaoyun;Xiong, Chunlan;Zhou, Xue;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Wang, Yong;Mao, Yahui;Liu, Fengquan
关键词：diffusible signal factor; Lysobacter enzymogenes; quorum sensing; Xanthomonas campestris
-
S-nitrosylation of a receptor-like cytoplasmic kinase regulates plant immunity
作者：Cui, Beimi;Liu, Fengquan;Cui, Beimi;Liu, Fengquan;Cui, Beimi;Pan, Qiaona;Loake, Gary J.;Cui, Wenqiang;Wang, Yiqin;Cui, Wenqiang;Yuan, Shuguang;Wang, Yiqin;Loake, Verity I. P.;Loake, Gary J.
关键词：
-
Oomycete Nudix effectors display WY-Nudix conformation and mRNA decapping activity
作者：Guo, Baodian;Wang, Bangwei;Wang, Haonan;Kong, Guanghui;Dong, Suomeng;Wang, Yuanchao;Guo, Baodian;Wang, Bangwei;Liu, Fengquan;Hu, Qinli;Yao, Deqiang;Han, Chenyang;Liu, Fengquan;Xing, Weiman
关键词：
-
Evaluating the Efficacy of Hymexazol in Controlling Globisporangium spinosum, the Newly Identified Pathogen Causing Root Rot in Houttuynia cordata
作者：Zhou, Xue;Xiong, Chunlan;Ma, Chaoyun;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Zhou, Xue;Xiong, Chunlan;Ma, Chaoyun;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Tu, Yupei;Wang, Yong;Li, Kaihuai;Liu, Fengquan
关键词：Houttuynia cordata; hymexazol; oomycete; root rot
-
Regulatory Effects of Diverse DSF Family Quorum-Sensing Signals in Plant-Associated Bacteria
作者：Li, Kaihuai;Ma, Chaoyun;Zhou, Xue;Xiong, Chunlan;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Ma, Chaoyun;Zhou, Xue;Xiong, Chunlan;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Wang, Yong;Wang, Bo;Liu, Fengquan
关键词：bacterial intraspecies regulation; diffusible signal factor (DSF); plant innate immunity; quorum sensing (QS)
-
Uncovering the mechanisms underlying pear leaf apoplast protein-mediated resistance against Colletotrichum fructicola through transcriptome and proteome profiling
作者：Han, Chenyang;Su, Zhiyuan;Wang, Qi;Zhang, Shaoling;Zhao, Yancun;Li, Chaohui;Guo, Baodian;Liu, Fengquan
关键词：Colletotrichum fructicola; Pear; Apoplast; Glycoside hydrolases; Plant immunity
