Computer-aided discovery of a novel thermophilic laccase for low-density polyethylene degradation
文献类型： 外文期刊
作者： Zhang, Yan 1 ; Plesner, Thea Jess 1 ; Ouyang, Yi 1 ; Zheng, Yu-Cong 2 ; Bouhier, Etienne 3 ; Berentzen, Emilie Ingemann 1 ; Zhang, Mingliang 1 ; Zhou, Pengfei 1 ; Zimmermann, Wolfgang 6 ; Andersen, Gregers Rom 7 ; Eser, Bekir Engin 1 ; Guo, Zheng 1 ;
作者机构： 1.Aarhus Univ, Dept Biol & Chem Engn, DK-8000 Aarhus, Denmark
2.Max Planck Inst Terr Microbiol, Karl von Fr Str 10, D-35043 Marburg, Germany
3.Univ Technol Compiegne, Dept Biol Engn, Compiegne, France
4.Fujian Normal Univ, Engn Res Ctr Ind Microbiol, Minist Educ, Fuzhou, Peoples R China
5.Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Key Lab Funct Foods, Guangdong Key Lab Agr Prod Proc,Minist Agr & Rural, Guangzhou 510610, Peoples R China
6.Univ Leipzig, Inst Analyt Chem, D-04103 Leipzig, Germany
7.Aarhus Univ, Dept Mol Biol & Genet, DK-8000 Aarhus, Denmark
关键词： Enzyme discovery; Laccase; Thermophilic enzyme; PE degradation; Dye decolorization
期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）
ISSN： 0304-3894
年卷期： 2023 年 458 卷
页码：
收录情况： SCI
摘要： Polyethylene (PE) and industrial dyes are recalcitrant pollutants calling for the development of sustainable solutions for their degradation. Laccases have been explored for removal of contaminants and pollutants, including dye decolorization and plastic degradation. Here, a novel thermophilic laccase from PE-degrading Lysinibaccillus fusiformis (LfLAC3) was identified through a computer-aided and activity-based screening. Biochemical studies of LfLAC3 indicated its high robustness and catalytic promiscuity. Dye decolorization experiments showed that LfLAC3 was able to degrade all the tested dyes with decolorization percentage from 39% to 70% without the use of a mediator. LfLAC3 was also demonstrated to degrade low-density polyethylene (LDPE) films after eight weeks of incubation with either crude cell lysate or purified enzyme. The formation of a variety of functional groups was detected using Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). Damage on the surfaces of PE films was observed via scanning electron microscopy (SEM). The potential catalytic mechanism of LfLAC3 was disclosed by structure and substrate-binding modes analysis. These findings demonstrated that LfLAC3 is a promiscuous enzyme that has promising potential for dye decolorization and PE degradation.
- 相关文献
作者其他论文 更多>>
-
Regulating heat-induced fibrous whey protein-wheat starch composite emulsion gels as dysphagia food by preheating temperature: Insights from protein-starch interactions
作者：Wang, Zhiming;Zeng, Jiarui;Deng, Yuanyuan;Zhou, Pengfei;Li, Ping;Zhao, Zhihao;Liu, Guang;Zhang, Mingwei;Wang, Zhiming;Zhang, Mingwei;Zhang, Mingwei
关键词：Texture-modified; IDDSI test; Protein aggregates; Gel network structure; Starch digestibility
-
Impact of homogenization methods on the interfacial protein composition and stability of peanut oil body emulsion with sodium caseinate and maltodextrin
作者：Lin, Zihui;Zhou, Pengfei;Deng, Yuanyuan;Liu, Guang;Li, Ping;Zeng, Jiarui;Zhang, Yan;Tang, Xiaojun;Zhao, Zhihao;Zhang, Mingwei;Lin, Zihui
关键词：Peanut oil body; Homogenization methods; Emulsion; Interfacial protein; Stability
-
Cyclic trace enzymatic hydrolysis pretreatment enhances brown rice: Cooking and taste
作者：Deng, Yuanyuan;Zhou, Pengfei;Li, Ya;Li, Ping;Zhao, Zhihao;Wang, Jiajia;Zhong, Lihuang;Liu, Guang;Zhang, Mingwei;Zeng, Jiarui;Liu, Dong
关键词：Brown rice; Cyclic trace enzymatic hydrolysis; Quality improvement
-
Modulation of Gut Mycobiome and Serum Metabolome by a MUFA-Rich Diet in Sprague Dawley Rats Fed a High-Fructose, High-Fat Diet
作者：Zhao, Zhihao;Zhong, Lihuang;Zeng, Guangzhen;Liu, Songbin;Deng, Yuanyuan;Zhang, Yan;Tang, Xiaojun;Zhang, Mingwei;Wu, Jiajin
关键词：dietary fatty acids; oleic acid; high-fat; high-fructose diet; gut fungi; bile acid metabolism; CoA biosynthesis
-
A Genome-Wide Analysis of the VuR2R3-MYB Gene Family in Cowpea and Its Expression in Anthocyanin Accumulation
作者：Yang, Yi;Zhou, Xuan;Wu, Zengxiang;Shen, Zhuo;Li, Tinyao;Zhang, Yan;Yang, Yi;Zhou, Xuan;Wu, Zengxiang;Shen, Zhuo;Li, Tinyao;Zhang, Yan;Yu, Canye
关键词：cowpea; anthocyanin accumulation
-
Black rice stepwise fermentation enhances yogurt gel quality: Solidification rate, texture, LAB distribution and viability persistence
作者：Zeng, Jiarui;Wang, Zhiming;Liu, Guang;Jia, Xuchao;Zhou, Pengfei;Li, Ping;Zhao, Zhihao;Zhang, Yan;Tang, Xiaojun;Deng, Yuanyuan;Zhang, Mingwei;Liu, Dong
关键词：Black rice; Stepwise fermentation; Yogurt; LAB distribution; LAB viability; Texture quality
-
Ultrastable high internal phase Pickering emulsions stabilized by deamidated pea protein: Formation mechanisms and applications in 3D printing
作者：Luo, Lijuan;Li, Ping;Deng, Yuanyuan;Liu, Guang;Zhang, Yan;Tang, Xiaojun;Zhou, Pengfei;Zhao, Zhihao;Zhang, Mingwei;Luo, Lijuan
关键词：Deamidated pea protein isolate; High internal phase emulsions; 3D printing; Rheological properties; Lipid digestion
