文献类型： 外文期刊

作者： Gong Lan ¹ ; Chen Ming ¹ ; Wei Xian ¹ ; Zou Chun ¹ ; Wang Ran ¹ ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Qual & Safety,Minist Agr, Key Lab Control Technol & Stand Agroprod Safety &, Jiangsu Key Lab Food Qual & Safety,State Key Lab, Nanjing 210014, Jiangsu, Peoples R China

关键词： Glucocorticoids;High performance liquid chromatography tandem mass spectrometry;Solid-phase extraction;Muscle tissues

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2016 年 44 卷 1 期

页码：

收录情况： SCI

摘要： A method was developed to determine cortisone, cortisol, prednisone and dexamethasone in muscle tissues by high performance liquid chromatography. tandem mass spectrometry (LC-ESI-MS/MS). Deconjugation of glucocorticoids was carried out by enzymatic hydrolysis. These preliminary steps were followed by ethyl acetate extraction and HLB solid phase extraction clean up step for all matrices. Chromatographic separation was achieved on C-8 column and MS/MS data were obtained in the multiple reaction monitoring mode using negative electrospray ionization. Calibration graphs were prepared in the 0. 5-50. 0 mu g/L range and good linearity was achieved (R-2 >= 0. 99). The limits of detection (LOQ) and the limits of quantification (LOD) were 0. 13-0. 25 mu g/kg and 0. 25-0. 50 mu g/kg, respectively. Spiking at the levels of 0. 5, 5. 0 and 10. 0 mu g/L, the average recovery for glucocorticoids ranged from 74. 0% to 101. 8%. The relative standard deviations (RSD) were between 0. 7%-8. 6%.