文献类型： 外文期刊

作者： Wei Rui-Cheng ¹ ; Li Guo-Feng ¹ ; Gao Rong ² ; Sun Ting ³ ; Gong Lan ¹ ; Chen Ming ¹ ; Wang Ran ¹ ;

作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Prov Key Lab Anim Derived Food Safety, Key Lab Control Technol & Standard Agroprod Safet, Minist Agricultrue, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Med Univ, Sch Publ Hlth, Dept Hyg Anal & Detect, Nanjing 210029, Jiangsu, Peoples R China

3.Nanjing Med Uni

关键词： High performance liquid chromatography-tandem mass spectrometry;Milk;Quaternary ammonium compounds;Residues

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2014 年 42 卷 5 期

页码：

收录情况： SCI

摘要： A method was proposed for the determination of the residues of the quaternary ammonium compounds (QACs) in milk by solid phase extraction. high performance liquid chromatography. tandem mass spectrometry (HPLC-MS/MS). The sample was extracted with acetonitrile, cleaned. up by weak cationic exchange solid phase extraction cartridge and concentrated in a gentle nitrogen stream. The separation of 5 QACs was performed on a Thermo C-18 column (150 mmx2. 1 mm, 3 mu m) using gradient elution of 0. 1% acetic acid and 20 mmol/L ammonium acetate methanol as the mobile phase within 7 min. The QACs were analyzed under the multiple reaction monitoring (MRM) mode with positive electrospray ionization. The calibration curves were linear well with correlation coefficient over 0. 99. The limit of quantification of the method was 1. 0 mu g/kg for hexadecyltrimethyl ammonium bromide and 0. 2 mu g/kg for benzyldodecyldimethyl ammonium bromide, tetradecyl trimethyl ammonium bromide, benzyldimethyltetradecyl ammonium chloride and didecyldimethyl ammonium bromide. The average recoveries for 5 QACs were between 70. 5% and 96. 6% in pure milk, between 69. 1% and 87. 9% in fresh milk and between 67. 1% and 93. 3% in low fat milk at the spiked levels of 2, 10 and 20 mu g/kg, with relative standard derivation (RSD) from 1. 4% to 11. 6%. The developed method is simple and sensitive, and suitable for rapid analysis of quaternary ammonium compounds in the milk.