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Overexpression of potato miR482e enhanced plant sensitivity to Verticillium dahliae infection

文献类型： 外文期刊

作者： Yang, Liu 1 ; Mu, Xiaoying 1 ; Liu, Chao 1 ; Cai, Jinghui 1 ; Shi, Ke 1 ; Zhu, Wenjiao 1 ; Yang, Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

关键词： miR482;nucleotide binding site leucine-rich repeat;potato;sRNA;ta-siRNA;Verticillium dahliae;Verticillium wilt

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2015 年 57 卷 12 期

页码：

收录情况： SCI

摘要： Verticillium wilt of potato is caused by the fungus pathogen Verticillium dahliae. Present sRNA sequencing data revealed that miR482 was in response to V. dahliae infection, but the function in potato is elusive. Here, we characterized potato miR482 family and its putative role resistance to Verticillium wilt. Members of the potato miR482 superfamily are variable in sequence, but all variants target a class of disease-resistance proteins with nucleotide binding site (NBS) and leucine-rich repeat (LRR) motifs. When potato plantlets were infected with V. dahliae, the expression level of miR482e was downregulated, and that of several NBS-LRR targets of miR482e were upregulated. Transgenic potato plantlets overexpressing miR482e showed hypersensitivity to V. dahliae infection. Using sRNA and degradome datasets, we validated that miR482e targets mRNAs of NBS-LRR disease-resistance proteins and triggers the production of trans-acting (ta)siRNAs, most of which target mRNAs of defense-related proteins. Thus, the hypersensitivity of transgenic potato could be explained by enhanced miR482e and miR482e-derived tasiRNA-mediated silencing on NBS-LRR-disease-resistance proteins. It is speculated that a miR482-mediated silencing cascade mechanism is involved in regulating potato resistance against V. dahliae infection and could be a counter defense action of plant in response to pathogen infection.

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[1]Genomic, evolutionary and expression profile analysis of Hsp70 superfamily in A and D genome of cotton (Gossypium spp.) under the challenge of Verticillium dahliae. Xiao, Songhua,Yu, Jingzhong,Yu, Deyue,Xiao, Songhua,Xu, Jianwen,Zhao, Jun,Liu, Jianguang,Wu, Qiaojuan. 2017

[2]Cloning and expression of a wild eggplant cytochrome P450 gene, StoCYP77A2, involved in plant resistance to Verticillium dahliae. Yang, Liu,Shi, Ce,Mu, Xiaoying,Liu, Chao,Shi, Ke,Zhu, Wenjiao,Yang, Qing,Yang, Liu.

[3]Ectopic expression of a Ve homolog VvVe gene from Vitis vinifera enhances defense response to Verticillium dahliae infection in tobacco. Tang, Juan,Lin, Jing,Chang, Youhong,Yang, Yuwen,Chen, Tianzi,Ling, Xitie,Zhang, Baolong,Chang, Youhong.

[4]Gbvdr6, a Gene Encoding a Receptor-Like Protein of Cotton (Gossypium barbadense), Confers Resistance to Verticillium Wilt in Arabidopsis and Upland Cotton. Yang, Yuwen,Ma, Zhengqiang,Yang, Yuwen,Ma, Zhengqiang,Yang, Yuwen,Chen, Tianzi,Ling, Xitie. 2018

[5]A Ve homologous gene from Gossypium barbadense, Gbvdr3, enhances the defense response against Verticillium dahliae. Chen, Tianzi,Kan, Jialiang,Yang, Yuwen,Ling, Xitie,Chang, Youhong,Zhang, Baolong.

[6]Two Lysin-Motif Receptor Kinases, Gh-LYK1 and Gh-LYK2, Contribute to Resistance against Verticillium wilt in Upland Cotton. Li, Fangfang,Qian, Shasha,Zhou, Xueping,Gu, Zhouhang,Wang, Qian,Ye, Fei,Liu, Tingli,Chen, Tianzi,Yang, Yuwen,Wang, Jinyan,Zhang, Baolong,Ding, Bo,Wang, Guoliang,Zhou, Xueping. 2017

[7]De Novo Sequencing and Analysis of the Transcriptome of the Wild Eggplant Species Solanum Aculeatissimum in Response to Verticillium dahliae. Zhou, Xiaohui,Bao, Shengyou,Liu, Jun,Zhuang, Yong.

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[9]Isolation and Characterization of Endophytic Bacillius subtilis Jaas ed1 Antagonist of Eggplant Verticillium Wilt. Lin, Ling,Dong, Han-Song,Lin, Ling,Qiao, Yong-Sheng,Ju, Zheng-Ying,Ma, Chang-Wen,Zhou, Yi-Jun,Liu, Ying-Hao.

[10]A Cotton Gbvdr5 Gene Encoding a Leucine-Rich-Repeat Receptor-Like Protein Confers Resistance to Verticillium dahliae in Transgenic Arabidopsis and Upland Cotton. Yang, Yuwen,Ling, Xitie,Chen, Tianzi,Cai, Liwang,Liu, Tingli,Wang, Jinyan,Fan, Xiaohui,Ren, Yongzhe,Yuan, Hongbo,Zhu, Wei,Zhang, Baolong,Ma, Din-Pow.

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