文献类型： 外文期刊

作者： Bahareldin-Ali, Abdalla ¹ ; Qin Guang-sheng ² ; Guo Ri-hong ¹ ; Tsigkou, Anastasia ³ ; Tan Zheng-zhun ² ; Jian, Hua ¹ ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

2.Guangxi Buffalo Res Inst, Key Lab Buffalo Genet Breeding & Reprod Technol, Nanning 530000, Peoples R China

3.Xian Jiaotong Liverpool Univ, Dept Biol Sci, Suzhou 215123, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Anim Sci, Lab Anim Breeding & Reprod, Nanjing 210014, Peoples R China

关键词： inhibin immunization;ovarian responses;luteal function;hormonal patterns;conception rate;water buffalo cows

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2015 年 14 卷 9 期

页码：

收录情况： SCI

摘要： The aim of this study was to investigate the feasibility of stimulating ovarian follicle development in order to improve fertility in water buffalo cows by immunization against inhibin. The experiment was carried out in early summer (May) and included 24 multi-parity crossbred Murrah-Swamp buffaloes that were divided into immunized (n=11) and control (n=13) groups. Each immunized cow was administered with a 2-mL immunogen of mineral oil adjuvant containing 2 mg of recombinant inhibin a-subunit fusion protein. The controls were treated with the adjuvant only. All animals received Ovsynch protocol treatment, starting on the day of the antigen administration, and they were artificially inseminated upon behavioral estrus. As a result, all of the immunized buffaloes generated antibodies against inhibin during the experimental period and had higher plasma concentrations of follicle-stimulating hormone (FSH), activin, and estradiol (E2) related to estrous expression. A higher proportion of immunized animals expressed estrus behavior than did the controls (72% vs. 30%, P<0.05). On average, inhibin-immunized buffaloes had significantly more large follicles mm in diameter) than the controls (mean SEM; 1.2 +/- 0.1 vs. 0.84 +/- 0.1, respectively; P<0.05) and a slightly higher mean total number of follicles (>= 2 mm; 11.4 +/- 0.7 vs. 9.0 +/- 1.1, respectively; P=0.09) and small (2-4 mm) follicles (8.81 +/- 0.6 vs. 6.84 +/- 1.0, respectively; P=0.12). A higher percentage of cows ovulated in the immunized group than in the control group (91% (10/11) vs. 54% (7/13), respectively; P<0.05). Moreover, inhibin-immunized cows had slightly larger corpus luteum (CL) than the controls 9 days after ovulation and significantly higher (P<0.01) post-ovulation peak plasma progesterone (P4) concentrations. Immunization against inhibin also marginally increased the conception rate 42 days after insemination (45:8% vs. 15.4%; P>0.05). These results demonstrate that immunization against inhibin, coupled with the treatment with the Ovsynch protocol, can constitute a new technique to increase fertility in water buffalo cows.