文献类型： 外文期刊
作者： Wen, Li-bin 1 ; Wang, Feng-zhi 1 ; He, Kong-wang 1 ; Li, Bin 1 ; Wang, Xiao-min 1 ; Guo, Rong-li 1 ; Xie, Jian-ping 2 ;
作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Minist Agr, Natl Ctr Engn Res Vet Bioprod,Inst Vet Med, Nanjing 210014, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Minist Agr, Natl Ctr Engn Res Vet Bioprod,Inst Vet
关键词： Porcine circovirus like virus P1;Transcriptional analysis;Northern blotting;RACE
期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）
ISSN： 1746-6148
年卷期： 2014 年 10 卷
页码：
收录情况： SCI
摘要： Background: Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome. In this study, we identified the viral genes and their corresponding mRNA transcripts. Results: The RNAs of P1, synthesized in porcine kidney cells, were examined with northern blotting and PCR analyses. Eight virus-specific RNAs were detected. Four mRNAs (open reading frames (ORFs) 1, 2, 4, and 5) are encoded by the viral (-) strand and four (ORFs 3, 6, 7, and 8) are encoded by the viral (+) strand. All proteins encoded by the ORFs of the P1 virus are less than 50 amino acids in length, except that encoded by ORF1 (113 amino acids). Conclusions: We show a very complex viral transcription pattern in P1-infected cells.
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