您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization of target mRNAs for Prunus persica microRNAs using an integrated strategy of PLM-RACE, PPM-RACE and qRT-PCR

文献类型： 外文期刊

作者： Zhang, Yanping 1 ; Han, Jian 1 ; Yu, Mingliang 2 ; Ma, Ruijuan 2 ; Pervaiz, Tariq 1 ; Fang, Jinggui 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Hort, Nanjing 210014, Jiangsu, Peoples R China

关键词： Ppe-miRNA;Target mRNA;PPM-RACE;RLM-RACE;Cleavage;qRT-PCR

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2014 年 170 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) in higher plants control their target mRNAs by either mRNA cleavage or translation repression at transcriptional or posttranscriptional levels. To verify more predicted target genes of Prunus persica microRNAs (Ppe-miRNAs) and elucidate the modes by which these Ppe-miRNAs work on their target genes, nine potential target genes (ppa005013m, ppa006611m, ppa007056m, ppa007653m, ppa001386m, ppa001781m, ppa003783m, ppa003465m, ppa004699m) for seven Ppe-miRNAs (PpemiR156a, Ppe-miR164a, Ppe-miR165a, Ppe-miR171a, Ppe-miR172a, Ppe-miR393a, Ppe-miR394a) were experimentally verified by a new strategy integrating modified 5' RLM-RACE (RNA ligase-mediated 5' rapid amplification of cDNA ends), 3' PPM-RACE (Poly(A) polymerase-mediated 3' rapid amplification of cDNA ends) and qRT-PCRs of cleavage products. The results show that these Ppe-miRNAs negatively regulate the expression of their target mRNAs by guiding cleavage of corresponding target mRNA with the cleavage sites mostly being at the tenth nucleotide of 5' end binding sites of Ppe-miRNAs. The expression patterns of Ppe-miRNAs, their target mRNAs and the cleavage fragments of target mRNAs were also analyzed in eight different peach tissues. There was an inverse relationship between expression of Ppe-miRNAs and their target mRNAs in eight tissues, and expression of both cleaved targets and Ppe-miRNAs verify the cleavage mode of Ppe-miRNAs on their target genes. Our results confirm the importance of Ppe-miRNAs in peach growth and development, and further increase understanding on the important regulatory roles of Ppe-miRNAs in peach, this suggest more study on Ppe-miRNAs and targets can enrich the knowledge of miRNA's functions in peach. (C) 2014 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Characterization of the miR165 family and its target gene Pp-ATHB8 in Prunus persica. Zhang, Chunhua,Guo, Lei,Ma, Ruijuan,Yu, Mingliang,Zhang, Yanping,Han, Jian,Li, Xiaoying. 2012

[2]Characterization of target mRNAs for grapevine microRNAs with an integrated strategy of modified RLM-RACE, newly developed PPM-RACE and qPCRs. Wang, Chen,Han, Jian,Korir, Nicholas Kibet,Leng, Xiangpeng,Fang, Jinggui,Wang, Xicheng,Liu, Hong,Li, Xiaoying.

[3]荷花淹水胁迫下实时定量PCR内参基因的验证. 徐君,江君,王欢,姜红卫. 2019

[4]茉莉花实时荧光定量PCR内参基因的筛选与验证. 齐香玉,陈双双,冯景,王华娣,邓衍明. 2020

[5]早熟砂梨‘苏翠1号’与其亲本‘翠冠’‘华酥’成熟果实差异代谢产物及差异基因比较分析. 王宏,杨王莉,蔺经,杨青松,李晓刚,盛宝龙,常有宏. 2022

[6]辣椒细胞质雄性不育相关基因的克隆与表达分析. 刘科伟,王述彬,刁卫平,戈伟. 2017

[7]辣椒胞质雄性不育相关基因的克隆及表达. 王述彬,刁卫平,刘金兵,潘宝贵,戈伟,郭广君. 2014

[8]不同激素处理下青花菜lncRNA内参基因的筛选. 冯鹏宇,裴徐梨,焦鹏,唐征,荆赞革,徐胜光,宋立晓,杞汝春,徐谦. 2025

[9]Genome-wide analysis of terpene synthases in soybean: Functional characterization of GmTPS3. Huang, Fang,Wang, Xia,Wang, Jiao,Yu, Deyue,Liu, Jianyu,Zhang, Man,Zheng, Rui.

[10]Selection of Reliable Reference Genes for Gene Expression Studies on Rhododendron molle G. Don. Xiao, Zheng,Sun, Xiaobo,Liu, Xiaoqing,Li, Chang,He, Lisi,Chen, Shangping,Su, Jiale. 2016

[11]Evaluation of appropriate reference genes for gene expression studies in pepper by quantitative real-time PCR. Bin, Wang Shu,Wei, Liu Ke,Ping, Diao Wei,Li, Zhi,Wei, Ge,Bing, Liu Jin,Gui, Pan Bao,Jian, Wan Hong,Feng, Chen Jin,Wei, Liu Ke.

[12]Computational identification of microRNAs in strawberry expressed sequence tags and validation of their precise sequences by miR-RACE. Han, Jian,Yu, Hua-Ping,Wang, Chen,Liu, Hong,Fang, Jing-Gui,Dong, Qing-Hua,Ge, An-Jing,Zhao, Mi-Zhen.

[13]Characterization of miRNAs responsive to exogenous ethylene in grapevine berries at whole genome level. Zhao, Fanggui,Wang, Chen,Han, Jian,Zhu, Xudong,Li, Xiaopeng,Fang, Jinggui,Zhao, Fanggui,Wang, Xicheng.

[14]Computational identification of microRNAs in peach expressed sequence tags and validation of their precise sequences by miR-RACE. Zhang, Yanping,Yu, Huaping,Han, Jian,Song, Changnian,Fang, Jinggui,Yu, Mingliang,Ma, Ruijuan.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台