Effects of pulsed electric fields on cytomembrane lipids and intracellular nucleic acids of Saccharomyces cerevisiae
文献类型: 外文期刊
作者: Zhao, Wei 1 ; Yang, Ruijin 1 ; Gu, Yanjie 1 ; Li, Chunyang 3 ;
作者机构: 1.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Farm Prod Proc, Nanjing 210014, Peoples R China
关键词: Pulsed electric fields (PEF);Sublethal injury;Cytomembrane;Nucleic acids
期刊名称:FOOD CONTROL ( 影响因子:5.548; 五年影响因子:5.498 )
ISSN: 0956-7135
年卷期: 2014 年 39 卷
页码:
收录情况: SCI
摘要: In this study, pulsed electric fields (PEF) treatments at 20 kV/cm for 0-500 mu s were applied to Saccharomyces cerevisiae to investigate the effects of PEF on the cytomembrane and intracellular nucleic acids. The change in composition of membrane lipids characterized by decrease in the ratio of contents of unsaturated fatty acids versus saturated fatty acids induced by PEF was observed, which was related with the increase in rigidness of cytomembrane (lower cytomembrane fluidity and higher cytomembrane viscosity) after PEE PEF treatment caused no significant change in DNA but disruption of RNA of S. cerevisiae, which was also confirmed by the increase of PEF resistance and no occurrence of injured microbial cells by supplementation of RNA stabilizer, magnesium. The results showed the damage of cytomembrane and RNA were associated with the sublethally injured S. cerevisiae, suggesting cytomembrane and RNA were primary targets for PEF induced microbial damage. (C) 2013 Elsevier Ltd. All rights reserved.
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