文献类型： 外文期刊

作者： Nasiru, Mustapha Muhammad ¹ ; Boateng, Evans Frimpong ² ; Alnadari, Fawze ³ ; Nassarawa, Sanusi Shamsudeen ⁵ ; Bassey, Anthony Pius ¹ ; Feng, Jin ¹ ; Song, Jiangfeng ¹ ; Masisi, Kabo ⁶ ; Bunhok, Taing ⁷ ; Roth, Chuon Mony ⁷ ; Sovath, Sun ⁷ ; Pharith, Hay ⁷ ; Yan, Wenjing ⁸ ; Zhang, Jianhao ⁸ ; Li, Chunyang ¹ ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210014, Peoples R China

2.Univ Energy & Nat Resources, Sch Agr & Technol, Dept Food Sci & Technol, Dormaa Ahenkro Campus,POB 214, Sunyani, Ghana

3.Res & Dev Ctr Jiangsu Tianmeijian Nat Bioengn Co L, Nanjing 210046, Peoples R China

4.Tianmeijian Biotechnol Beijing Co Ltd, Native Nutr & Med Res Inst, Beijing 100101, Peoples R China

5.Bayero Univ, Fac Agr, Dept Food Sci & Technol, Kano, Kano, Nigeria

6.Botswana Int Univ Sci & Technol, Fac Sci, Dept Biol Sci & Biotechnol, Palapye, Botswana

7.China Cambodia Joint Lab Detect & Funct Evaluat Nu, Phnom Penh 10103, Cambodia

8.Nanjing Agr Univ, Coll Food Sci & Technol, Natl Ctr Meat Qual & Safety Control, Collaborat Innovat Ctr Meat Prod & Proc Qual & Saf, Nanjing 210095, Peoples R China

关键词： Nonthermal plasma (NTP); Quasi-targeted metabolomics; Peanut; Antioxidant metabolism; Amino acid biosynthesis; Energy metabolism

期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）

ISSN： 0308-8146

年卷期： 2025 年 490 卷

页码：

收录情况： SCI

摘要： Nonthermal plasma (NTP) is a promising food processing technology that enhances food safety and quality while preserving nutrition. This study used a novel quasi-targeted metabolomics approach to analyze NTP's effects on peanuts (Arachis hypogaea) under three treatment conditions (90, 270, 450 s at 160 kV, 150 Hz) and a control. The quasi-targeted method combines the strengths of targeted and untargeted metabolomics, offering high throughput, extensive coverage, and increased sensitivity. Of 1175 identified metabolites, 247 were significantly altered (135 upregulated, 112 downregulated). Key findings include upregulation of antioxidants like ascorbic acid (+23 %) and naringenin (+18 %) and markers of lipid peroxidation such as malondialdehyde (+42 %) and 4-hydroxy-2-nonenal (+30 %), indicating oxidative stress. Notably, Sulfur-containing amino acids, such as Lcysteine (+15 %) and homocysteine (+18 %), emerged as novel biomarkers for NTP-induced oxidative stress. Moderate treatment (270 s) enhanced antioxidant levels and maintained membrane integrity, whereas prolonged exposure (450 s) caused excessive oxidative stress, leading to nutrient degradation and protein oxidation. PCA and pathway analysis revealed changes in energy metabolism, amino acid biosynthesis, and secondary metabolites. These findings underscore NTP's potential to enhance peanut nutritional quality and shelf life, but the treatment must be optimized to balance its benefits with oxidative damage. Additionally, this study introduces sulfur-containing amino acids as innovative biomarkers for oxidative stress, offering new directions for monitoring NTP treatment effects and guiding industrial applications.