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Synthesis of Epoxy-functionalized Micro-zone Plates by UV-initiated Copolymerization

文献类型： 外文期刊

作者： Li, Li 1 ; Wang, He-Ye 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Facil & Equipment Agr, Nanjing 210014, Jiangsu, Peoples R China

关键词： UV-initiated copolymerization;Micro-zone plates;Epoxy

期刊名称：MATERIALS, MACHINES AND DEVELOPMENT OF TECHNOLOGIES FOR INDUSTRIAL PRODUCTION

ISSN： 1660-9336

年卷期： 2014 年 618 卷

页码：

收录情况： SCI

摘要： As microfluidic systems transition from research tools to disposable clinical devices, new substrate materials are need to meet both the regulatory requirement as well as the economics of disposable devices. In this paper, a commercial ultraviolet (UV)-curiable material (bisphenol A based epoxy acrylate, BABEA) was introduced as a new manufacturing material for facile fabrication of epoxy-functionalized microfluidic devices by UV-initiated copolymerization. X-ray photoelectron spectroscope (XPS) results indicated the existence of epoxy groups on the surface of poly (BABEA-co-GMA), which allowed for binding protein through an epoxy-amino group reaction. Poly (BABEA-co-GMA) is highly transparent in visible range, and of high replication fidelity. A fabrication procedure was proposed for manufacturing BABEA based epoxy-functionalized micro-zone plates. The fabrication procedure was very simple; obviating the need of micromachining equipments, wet etching or imprinting techniques. To evaluate the BABEA based epoxy-functionalized micro-zone plates, a-fetoprotein (AFP) antibody was immobilized onto the capture zone for chemiluminescent (CL) detection in a non-competitive immune response format. The proposed AFP immunoaffinity micro-zone plate was demonstrated as a low cost, flexible, homogeneous and stable assay for AFP.

  • 相关文献

[1]Paper-based immune-affinity arrays for detection of multiple mycotoxins in cereals. Li, Li,Chen, Hongpu,Lv, Xiaolan,Wang, Min,Jiang, Xizhi,Jiang, Yifei,Wang, Heye,Zhao, Yongfu,Xia, Liru,Li, Li,Lv, Xiaolan,Chen, Hongpu,Jiang, Yifei. 2018

[2]Synthesis of hydrophilic epoxy-functionalized films by UV-initiated copolymerization. Li, Li,Feng, Min,Zhu, Jiating. 2013

[3]Development of immune-affinity 96 spots monolith array for multiple mycotoxins detection in food samples. Li, Li,Xia, Li-Ru,Zhao, Yong-Fu,Wang, He-Ye.

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