您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of a loop-mediated isothermal amplification assay for the detection of porcine hokovirus

文献类型： 外文期刊

作者： Li, Bin 1 ; Sun, Bing 1 ; Du, Lu-ping 1 ; Mao, Ai-hua 1 ; Wen, Li-bin 1 ; Ni, Yan-xiu 1 ; Zhang, Xue-han 2 ; He, Kong-wan 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med,Minist Agr, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med,Minist

关键词： Porcine hokovirus;Loop-mediated isothermal amplification;PCR;Detection

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2013 年 193 卷 2 期

页码：

收录情况： SCI

摘要： Hokoviruses have recently been detected as pathogens belonging to the family Parvoviridae, which comprises porcine hokovirus (PHoV) and bovine hokovirus (BHoV). In this study, we developed a loopmediated isothermal amplification (LAMP) assay for the rapid, specific and sensitive detection of PHoV. A set of four primers specific for six regions within the PHoV VP1/2 genes was designed using online software. The reaction temperature and time were optimized at 65 degrees C and 60 min, respectively. LAMP products were detected by agarose gel electrophoresis or by visual inspection of a color change caused by a fluorescent dye. The method was highly specific for PHoV, and no cross-reaction was observed with porcine circovirus type 2 (PCV2), porcine parvovirus (PPV), porcine bocavirus (PBoV), pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV), classic swine fever virus (CSFV), or Japanese encephalitis virus (JEV). The detection limit was approximately 10 copies per reaction, which was 10 times more sensitive than conventional PCR. Furthermore, the efficiency of detection of PHoV in clinical samples was comparable to that of PCR and sequencing. These results show that the LAMP assay is a simple, rapid, sensitive and specific method for detecting PHoV. It does not require specialized equipment and can be used to detect PHoV both in the laboratory and in the field. (C) 2013 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Mycoplasma hyorhinis. Du, Gai-Mei,Liu, Mao-Jun,Wu, Yu-Zi,Xiong, Qi-Yan,Bai, Fang-Fang,Feng, Zhi-Xin,Shao, Guo-Qing,Du, Gai-Mei. 2013

[2]Development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus. Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Xiao, Shao-bo. 2012

[3]Rapid and Sensitive Detection of Didymella bryoniae by Visual Loop-Mediated Isothermal Amplification Assay. Yao, Xiefeng,Li, Pingfang,Xu, Jinghua,Zhang, Man,Ren, Runsheng,Liu, Guang,Yang, Xingping. 2016

[4]A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene. Liu, M. J.,Du, G. M.,Bai, F. F.,Wu, Y. Z.,Xiong, Q. Y.,Feng, Z. X.,Li, B.,Shao, G. Q.,Du, G. M.,Liu, M. J.,Shao, G. Q.. 2015

[5]IMMOBILIZATION OF DYE-DECOLORIZING PEROXIDASE ON MAGNETIC NANOPARTICLES: A DUAL-FUNCTIONAL BIOCATALYST FOR MYCOTOXINS DEGRADATION AND HYDROGEN PEROXIDE DETECTION. Yu Xia,Yangyu Qiu,Yang You,Lili Zhang,Zhouping Wang. 2023

[6]农杆菌介导rolC基因转化葡萄砧木'5BB'的研究. 李金凤,杨丽娜,周蓓蓓,章镇,李国平,陶建敏. 2007

[7]猪链球菌2型的快速诊断与毒力因子分子生物学研究. 何孔旺,倪艳秀,徐淑菲,王继春,俞正玉,何家惠,陆承平,姚火春. 2003

[8]Prevalence of porcine circovirus-like agent P1 in Jiangsu, China. Wen, Libin,He, Kongwang,Yu, Zhengyu,Mao, Aihua,Zhong, Shulin,Ni, Yanxiu,Zhang, Xuehan,Li, Bin,Wang, Xiaomin,Zhou, Junming,Guo, Rongli,Lv, Lixin,Jiang, Jieyuan,Yang, Hanchun. 2011

[9]ALTERATIONS OF DNA METHYLATION IN DIVERSE GRAFTED HYBRID TOMATOES (Solanum lycopersicum L.). Xu Xincheng,Song Weiyi,Ding Jinping,Ali, Zulfiqar,Ghori, Nida,Ali, Zulfiqar,Shao Hongbo,Shao Hongbo.

[10]Aptamer-Based Fluorescence Assay for Hg2+ Determination. Lei Zhao-Jing,Hu Qiu-Hui,Lei Zhao-Jing,Zhang Cun-Zheng,Liu Yuan,Zhang Qiang,Liu Xian-Jin. 2012

[11]Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR. Guo, Yingpeng,Li, Wei,Sun, Haiyan,Wang, Ning,Chen, Huaigu,Guo, Yingpeng,Yu, Hanshou. 2012

[12]Fluorescence polarization assay for the simultaneous determination of bisphenol A, bisphenol F and their diglycidyl ethers in canned tuna. Guan, Tianzhu,Li, Tiezhu,Li, Zhuolin,Wang, Yongzhi,Zhang, Jie,Wang, Yongjun,Zhang, Tiehua,Yu, Hansong,Ruan, Ping. 2017

[13]Selection of a Single Chain Variable Fragment Antibody against Ivermectin from a Phage Displayed Library. Wen, Shuang,Zhang, Xiao,Liu, Yuan,Zhang, Qingqing,Liu, Xianjin,Wen, Shuang,Liang, Jiansheng.

[14]Reverse transcription loop-mediated isothermal amplification of RNA for sensitive and rapid detection of southern rice black-streaked dwarf virus. Zhou, Tong,Du, Linlin,Fan, Yongjian,Zhou, Yijun,Zhou, Tong,Du, Linlin,Zhou, Yijun.

[15]大豆携带菜豆晕疫病菌PCR检测技术研究. 杨万风,刘艳,刘翔,邵沛泽,谌运清,赵文军. 2014

[16]氨基化磁性纳米粒子捕获基因组DNA结合PCR快速检测牛奶中金黄色葡萄球菌. 崔妍,白亚龙,施春雷,王大鹏,史贤明. 2017

[17]不同地区小麦纹枯病菌的生物学特性及种类鉴定研究. 陈健华,邢锦城,张茸茸,张旭,陈怀谷,马鸿翔. 2010

[18]洋葱细胞质雄性不育系101A的分子鉴定. 陈沁滨,侯喜林,王建军,冷月强,蒋芳玲,薛萍. 2006

[19]草鸡J亚群禽白血病病毒的PCR检测. 张敬峰,张晓勇,赵毓郎,魏雪涛,李银,刘宇卓. 2010

[20]黄淮麦区部分小麦品种(系)1Dx5+1Dy10的分子检测与分析. 王静,刘东涛,冯国华,赵军海,陈荣振. 2010

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台