Identification and Characterization of a Novel Monoterpene Synthase from Soybean Restricted to Neryl Diphosphate Precursor
文献类型： 外文期刊
作者： Zhang, Man 1 ; Liu, Jianyu 1 ; Li, Kai 1 ; Yu, Deyue 1 ;
作者机构： 1.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing, Jiangsu, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2013 年 8 卷 10 期
页码：
收录情况： SCI
摘要： Terpenes are important defensive compounds against herbivores and pathogens. Here, we report the identification of a new monoterpene synthase gene, GmNES, from soybean. The transcription of GmNES was up-regulated in soybean plants that were infested with cotton leafworm (Prodenia litura), mechanically wounded or treated with salicylic acid (SA). Gas chromatography-mass spectrometry (GC-MS) analysis revealed that recombinant GmNES enzyme exclusively produced nerol, generated from a newly identified substrate for monoterpene synthase: neryl diphosphate (NPP). This finding indicates that GmNES is a nerol synthase gene in soybean. Subcellular localization using GFP fusions showed that GmNES localized to the chloroplasts. Transgenic tobacco overexpressing GmNES was generated. In dual-choice assays, the GmNES-expressing tobacco lines significantly repelled cotton leafworm. In feeding tests with transgenic plants, the growth and development of cotton leafworm were significantly retarded. This study confirms the ecological role of terpenoids and provides new insights into their metabolic engineering in transgenic plants.
- 相关文献
作者其他论文 更多>>
-
Cucumber Auxin Response Factor CsARF10a Regulates Leaf Morphogenesis and Parthenocarpic Fruit Set in Tomato
作者：Xu, Jian;Yao, Xiefeng;Lou, Lina;Zhang, Man;Liu, Guang;Yang, Xingping;Liu, Jinqiu;Zhu, Lingli;Hou, Qian;Xu, Jinhua;Zhu, Pinyu;Meng, Yongjiao;Li, Ji
关键词：ARFs; cucumber; CsARF10a; parthenocarpic fruit set
-
A Transcriptomic Analysis of Bottle Gourd-Type Rootstock Roots Identifies Novel Transcription Factors Responsive to Low Root Zone Temperature Stress
作者：Liu, Jinqiu;Zhang, Man;Xu, Jian;Yao, Xiefeng;Lou, Lina;Hou, Qian;Zhu, Lingli;Yang, Xingping;Liu, Guang;Xu, Jinhua;Liu, Jinqiu;Zhang, Man;Xu, Jian;Yao, Xiefeng;Lou, Lina;Hou, Qian;Zhu, Lingli;Yang, Xingping;Liu, Guang;Xu, Jinhua
关键词：bottle gourd; root; low root zone temperature; RNA-Seq; transcription factor
-
Detection of Candidate Loci and Genes Related to Phosphorus Efficiency at Maturity through a Genome-Wide Association Study in Soybean
作者：Wang, Qing;Yu, Wenqing;Zhao, Wei;Huang, Fang;Yu, Deyue;Wang, Hui;Cheng, Hao;Wang, Qing;Ning, Lihua
关键词：soybean; P efficiency; traits at maturity; GWAS; GmABCG39
-
Haploid induction and plant production in bottle gourd by pollination with gamma irradiated pollen
作者：Zhao, Qing;Zhang, Man;Qi, Han;Yang, Xingping;Zhao, Qing;Zhang, Man
关键词：Bottle gourd; Haploid; Irradiation; Parthenogenesis
-
Characterization of Fusarium root rot disease in grafted watermelon
作者：Zhang, Man;Yang, Xingping;Xu, Jinhua;Liu, Guang;Yao, Xiefeng;Ren, Runsheng
关键词：Fusarium oxysporum; Grafted watermelon; Resistance; Rootstock; Root rot
-
Proteomic Analysis of Fusarium oxysporum-Induced Mechanism in Grafted Watermelon Seedlings
作者：Zhang, Man;Xu, Jinhua;Ren, Runsheng;Liu, Guang;Yao, Xiefeng;Lou, Lina;Xu, Jian;Yang, Xingping;Zhang, Man;Ren, Runsheng
关键词：bottle gourd; Citrullus lanatus; Fusarium oxysporum f; sp; niveum; proteomics; rootstock grafting
-
Genome-wide identification and analysis of promising GDSL-type lipases related to gummy stem blight resistance in watermelon (Citrullus lanatus)
作者：Ren, Runsheng;Yang, Xingping;Xu, Jinhua;Zhang, Man;Liu, Guang;Yao, Xiefeng;Lou, Lina;Xu, Jian;Zhu, Lingli;Hou, Qian;Ren, Runsheng;Zhang, Keyun
关键词：Citrullus lanatus; GDSL family; Gummy stem blight; Phylogenetic analysis; Genome-wide identification
