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Characterization of the miR165 family and its target gene Pp-ATHB8 in Prunus persica

文献类型： 外文期刊

作者： Zhang, Chunhua 1 ; Zhang, Yanping 2 ; Guo, Lei 1 ; Han, Jian 2 ; Li, Xiaoying 2 ; Ma, Ruijuan 1 ; Yu, Mingliang 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Hort, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

关键词： Prunus persica;microRNA;miR165;Pp-ATHB8;Cleavage;miR-RACE;qRT-PCR

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2012 年 146 卷

页码：

收录情况： SCI

摘要： A study to identify and validate Prunus persica microRNAs belonging to the ppe-miR165 family was conducted using miR-RACE where the expression of these miRNAs could be detected in leaves, flower buds, flowers and fruits of P. persica by quantitative real-time PCR (qRT-PCR) with some members of this miRNA family exhibiting tissue-specific expression. In addition, Pp-ATHB8 which is one of the potential target genes for ppe-miR165 family was experimentally verified by PPM-RACE and RLM-RACE, from which the cleavage sites of the target mRNA were mapped and the expression patterns of cleaved fragments also detected, thus demonstrating the mode by which ppe-miR165 regulates the target gene Pp-ATHB8. Furthermore, spatiotemporal expression levels of the target gene and its cleaved fragments were analyzed by qRT-PCR, where they were found to exhibit expression trends at variance from ppe-miR165, thus indicating the cleavage mode of this miRNA on its target gene. The characterization of this important miRNA and its interaction with an equally important target gene in P. persica, further deepens our understanding of the role of ppe-miR165 in P. persica and expands the knowledge of small RNA-mediated regulation in this fruit crop. (C) 2012 Elsevier B.V. All rights reserved.

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