Disease-resistant transgenic adzuki bean plants obtained through an efficient transformation system
文献类型： 外文期刊
作者： Chen, Huatao 1 ; Chen, Xin 1 ; Gu, Heping 1 ; Yuan, Xingxing 1 ; Zhang, Hongmei 1 ; Cui, Xiaoyan 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing 210014, Peoples R China
关键词： epicotyls;regeneration;Vigna angularis;co-cultivation
期刊名称：CROP & PASTURE SCIENCE （ 影响因子：2.286； 五年影响因子：2.507 ）
ISSN： 1836-0947
年卷期： 2012 年 63 卷 11-12 期
页码：
收录情况： SCI
摘要： An efficient regeneration and transformation system was established and optimised for adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi). 6-Benzylaminopurine at 5 mg L-1 was used to increase adventitious bud induction frequency. The highest frequency of shoot elongation was 92.8% when using a medium composition of MS salts combined with 0.1 mg L-1 of IAA, 0.5 mg L-1 of GA(3), 1.0 mg L-1 of zeatin-riboside, 50 mg L-1 of aspartic acid, and 50 mg L-1 of glutamic acid. In vitro rooting was 100% when shoots were cultured on the solid MS medium supplemented with 1.0 mg L-1 of NAA. Reproducible transformation of epicotyl explants was developed using the A. tumefaciens EHA105 strain. Using a concentration of 40 mg L-1 of acetosyringone, 20 mM MES, and 5 mg L-1 of 6-benzylaminopurine in the co-cultivation medium, a transformation efficiency of 12.6% was attained. Using this transformation protocol, we obtained transgenic adzuki bean plants resistant to soybean mosaic virus by introducing the V. angularis VaPR3 gene.
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