Characterization of a Wheat Heme Oxygenase-1 Gene and Its Responses to Different Abiotic Stresses
文献类型: 外文期刊
作者: Xu, Dao-kun 1 ; Jin, Qi-jiang 1 ; Xie, Yan-jie 1 ; Liu, Ya-hui 1 ; Lin, Yu-ting 1 ; Shen, Wen-biao 1 ; Zhou, Yi-jun 2 ;
作者机构: 1.Nanjing Agr Univ, Cooperat Demonstrat Lab Centrifuge Tech, Coll Life Sci, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China
关键词: abiotic stress responses;carbon monoxide;heme oxygenase-1;prokaryotic expression in E. coli;wheat
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )
ISSN: 1422-0067
年卷期: 2011 年 12 卷 11 期
页码:
收录情况: SCI
摘要: In animals and recently in plants, heme oxygenase-1 (HO1) has been found to confer protection against a variety of oxidant-induced cell and tissue injuries. In this study, a wheat (Triticum aestivum) HO1 gene TaHO1 was cloned and sequenced. It encodes a polypeptide of 31.7 kD with a putative N-terminal plastid transit peptide. The amino acid sequence of TaHO1 was found to be 78% similar to that of maize HO1. Phylogenetic analysis revealed that TaHO1 clusters together with the HO1-like sequences in plants. The purified recombinant TaHO1 protein expressed in Escherichia coli was active in the conversion of heme to biliverdin IXa (BV), and showed that the V-max was 8.8 U.mg(-1) protein with an apparent K-m value for hemin of 3.04 mu M. The optimum Tm and pH were 35 degrees C and 7.4, respectively. The result of subcellular localization of TaHO1 showed that the putative transit peptide was sufficient for green fluorescent protein (GFP) to localize in chloroplast and implied that TaHO1 gene product is at least localized in the chloroplast. Moreover, we found that TaHO1 mRNA could be differentially induced by the well-known nitric oxide (NO) donor sodium nitroprusside (SNP), gibberellin acid (GA), abscisic acid (ABA), hydrogen peroxide (H2O2) and NaCl treatments. Therefore, the results suggested that TaHO1 might play an important role in abiotic stress responses.
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