Characterization of siRNAs derived from rice stripe virus in infected rice plants by deep sequencing
文献类型： 外文期刊
作者： Yan, Fei 1 ; Zhang, Hengmu 1 ; Adams, Michael J. 2 ; Yang, Jian 1 ; Peng, Jiejun 1 ; Antoniw, John F. 2 ; Zhou, Yijun; 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China
2.Rothamsted Res, Dept Plant Pathol & Microbiol, Harpenden AL5 2JQ, Herts, England
3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2010 年 155 卷 6 期
页码：
收录情况： SCI
摘要： RNA interference is a natural defense against viruses in plants. To date, the only viral siRNAs characterized have been those for positive-sense RNA viruses with one or two genome components. Here, we characterized siRNAs derived from rice stripe virus (RSV), a member of the genus Tenuivirus with four genomic RNAs and an ambisense coding strategy. Deep sequencing of small RNAs from infected rice leaves showed that siRNAs were derived almost equally from virion and complementary RNA strands and were mostly 20-22 nucleotides long. Most viral siRNAs were produced within the coding sequences and 5' termini of the RSV genome. RSV siRNAs had a higher G and lower C content than the viral genome but a strong A/U bias at the first nucleotide and a U bias at the final one, suggesting preferential targeting of such sequences by rice Dicer-like proteins.
- 相关文献
作者其他论文 更多>>
-
Research on mixed sample test for the rapid detection of rice stripe virus and southern rice black-streaked dwarf virus in vector
作者：Du, Linlin;Li, Tao;Wang, Yunyue;Du, Linlin;Li, Tao;Li, Chenyang;Lan, Ying;Lin, Feng;Zhou, Yijun;Zhou, Tong;Guo, Wei;Xu, Kai;Kundu, Jiban Kumar;Du, Linlin;Zhou, Tong
关键词：Mixed sample test; RSV; SRBSDV; Sentinel surveillance; SBPH
-
The miR-184-3p promotes rice black-streaked dwarf virus infection by suppressing Ken in Laodelphax striatellus (Fallén)
作者：Wu, Wei;Wang, Man;Deng, Zhiting;Xi, Minmin;Dong, Yan;Wang, Haitao;Zhang, Jianhua;Zhou, Yijun;Xu, Qiufang;Wu, Wei;Deng, Zhiting;Xi, Minmin;Wang, Changchun;Xu, Qiufang
关键词：miR-184-3p; Laodelphax striatellus; rice black-streaked dwarf virus; Ken; virus infection
-
Resistance gene Ty-1 restricts TYLCV infection in tomato by increasing RNA silencing
作者：Ma, Xiaofang;Wu, Liming;Ma, Xiaofang;Zhou, Yijun;Ma, Xiaofang;Moffett, Peter
关键词：RNA silencing; Nicotiana benthamiana; Ty-1; RDRs proteins; TYLCV V2
-
The miR184-3p targets neuron-specific ecdysone inducible protein 78 to promote rice black streaked dwarf virus propagation in its planthopper vector
作者：Wang, Haitao;Dong, Yan;Wang, Man;Li, Shuo;Zhou, Yijun;Ji, Yinghua
关键词：miR184-3p; rice black streak dwarf virus; antiviral mechanism; central nervous system; ecdysone inducible protein 78; ecdysone receptor
-
Geminivirus C4/AC4 proteins hijack cellular COAT PROTEIN COMPLEX I for chloroplast targeting and viral infections
作者：Zhao, Wenhao;Ji, Yinghua;Zhou, Yijun;Zhao, Wenhao;Wang, Xiaofeng
关键词：
-
Molecular Hydrogen Confers Resistance to Rice Stripe Virus
作者：Shao, Yudong;Lin, Feng;Wang, Zhaoyun;Liu, Zhiyang;Chen, Dongyue;Guo, Wei;Lan, Ying;Du, Linlin;Zhou, Yijun;Zhou, Tong;Shao, Yudong;Wang, Yueqiao;Cheng, Pengfei;Lou, Wang;Chen, Dongyue;Shen, Wenbiao
关键词：Arabidopsis; disease resistance; molecular hydrogen; rice; rice stripe virus; salicylic acid
-
Genetic variability of rice stripe virus after its pandemic in Jiangsu
作者：Lu, Chengye;Miu, Qian;Jin, Daoran;Cheng, Zhaobang;Zhou, Yijun;Li, Shuo;Lu, Chengye;Wang, Yunyue;Li, Aiguo
关键词：Rice stripe virus; Genetic variability; Non-epidemic period; Genotype; NSvc4 variation
