文献类型： 外文期刊
作者： Cai, Xiao 1 ; Kang, Xiang-Yang 1 ;
作者机构： 1.Beijing Forestry Univ, Minist Educ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Key Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Minist Educ,Inst Cotton, Key Lab Genet & Breeding Forest Trees & Ornamenta, Beijing Forestry Univ,Key Natl Engn Lab Tree Bree, Shijiazhuang 050051, Peoples R China
关键词： Plant regeneration;Cell suspension;Protoplast culture;Populus x beijingensis
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）
ISSN： 1054-5476
年卷期： 2014 年 50 卷 1 期
页码：
收录情况： SCI
摘要： A protocol for plant regeneration from cell suspension-derived protoplasts of Populus x beijingensis is described. Protoplasts were isolated from cell suspension cultures 6 d after subculture and further cultured in liquid NH4NO3-free Murashige and Skoog (MS) medium supplemented with 0.6 M glucose, 9.05 mu M 2,4-dichlorophenoxyacetic acid, and 0.89 mu M 6-benzyladenine at a density of 2 x 10(5) protoplasts per milliliter. The initial plating efficiency and final plating efficiency recorded after 10 and 30 d reached 33.7 and 1.07%, respectively. The proliferated calli transferred to regeneration medium supplemented with 2.22 mu M 6-benzyladenine and 0.54 mu M alpha-naphthaleneacetic acid gave the highest rate of shoot formation (44.4%). All protoplast-derived shoots were able to form roots on half-strength MS medium supplemented with 2.46 mu M indole-3-butyric acid.
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