文献类型： 外文期刊

作者： Ma Hong-Fang ¹ ; Qiao Song-Lin ¹ ; Huang Ming-Dong ³ ; Jiang Long-Guang ³ ; Li Rui ¹ ; Zhang Gai-Ping ¹ ;

作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Henan, Peoples R China

2.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China

3.Fuzhou Univ, Coll Chem, Fuzhou 350116, Fujian, Peoples R China

关键词： CD163;SRCR;SRCR-SF;X-ray crystallography

期刊名称：CHINESE JOURNAL OF STRUCTURAL CHEMISTRY （ 影响因子：0.893； 五年影响因子：0.603 ）

ISSN： 0254-5861

年卷期： 2017 年 36 卷 9 期

页码：

收录情况： SCI

摘要： CD163 is an endocytic receptor specifically expressed in monocytes/macrophages and belongs to class B of the scavenger receptor cysteine-rich (SRCR) superfamily (SRCR-SF). It plays critical roles in various physiological and pathological pathways. In this study, the fifth SRCR domain (SRCR5) of porcine CD163 (pCD163) was expressed in Pichia pastoris system. The recombinant protein was homogeneous after purification by metal-affinity and size-exclusion chromatography. The purified protein was then assayed for its biological function. Single crystals of pCD163 SRCR5 were obtained under the condition of 25% PEG 4000, 0.1 M citric acid pH3.5 and its structure was finally determined at 1.80 angstrom. All these results provide functional pCD163 SRCR5 in large quantity for future research, and enrich the structural details for CD163 and the SRCR-SF.