Development of a Synthetic Peptide ELISA Assay for the Detection of Foot-and-Mouth Disease Virus Nonstructural Protein Antibodies
文献类型: 外文期刊
作者: Yang Ji-fei 1 ; Yang Su-zhen 1 ; Yang Yan-yan 1 ; Zhi Ai-min 1 ; Zhao Dong 1 ; Zhi Yu-bao 1 ; Xing Guang-xu 1 ; Deng Rui- 1 ;
作者机构: 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
2.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Peoples R China
关键词: foot and mouth disease virus (FMDV);ELISA;synthetic peptide
期刊名称:AGRICULTURAL SCIENCES IN CHINA ( 影响因子:0.82; 五年影响因子:0.997 )
ISSN: 1671-2927
年卷期: 2010 年 9 卷 11 期
页码:
收录情况: SCI
摘要: In order to develop an ELISA assay with synthetic peptides for the detection of antibody to the nonstructural proteins of foot-and-mouth disease virus, specific peptides were synthesized by a solid-phase method according to FMDV NSPs B-cell epitopes, and were conjugated to carrier protein BSA. An ELISA system was developed to detect FMDV NSPs antibody with the conjugated proteins as the coating antigen. The optimal coating concentration of the antigen was determined as 2.5 mu g mL(-1). The comparative study of this assay with UBI NSP ELISA kit and national commercial 3ABC ELISA kit in the detection of 199 serum samples showed that they were very coincident, and the identity rates were 96.48 and 97.48%, respectively. The development of ELISA using the synthetic peptides as coating antigen is specific, reproducible, stable, and easy, and can be used to differentiate FMDV infected pigs from immunized pigs.
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