您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and characterization of long non-coding RNAs in subcutaneous adipose tissue from castrated and intact full-sib pair Huainan male pigs

文献类型： 外文期刊

作者： Wang, Jing 1 ; Hua, Liushuai 1 ; Chen, Junfeng 1 ; Zhang, Jiaqing 1 ; Bai, Xianxiao 1 ; Gao, Binwen 1 ; Li, Congjun; S 1 ;

作者机构： 1.Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Henan Key Lab Farm Anim Breeding & Nutr Regulat, 116 Huayuan Rd, Zhengzhou 450002, Henan, Peoples R China

2.Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Henan Key Lab Farm Anim Breeding & Nutr Regulat, 116 Huayuan

关键词： Subcutaneous adipose tissue;LncRNA;Pig;Fat deposition

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2017 年 18 卷

页码：

收录情况： SCI

摘要： Background: Long non-coding RNAs (lncRNAs) regulate adipose tissue metabolism, however, their function on testosterone deficiency related obesity in humans is less understood. For this research, intact and castrated male pigs are the best model animal because of their similar proportional organ sizes, cardiovascular systems and metabolic features. Results: We identified lncRNAs in subcutaneous adipose tissue by deep RNA-sequencing using the intact and castrated Huainan male pigs. The results showed that castration reduced serum testosterone but increased body fatness-related traits (serum triglyceride levels, backfat thickness, intramuscular fat content, and adipocyte size). Meanwhile, 343 lncRNAs from subcutaneous adipose tissue were identified, including 223 intergenic lncRNAs (lincRNAs), 68 anti-sense lncRNAs, and 52 intronic lncRNAs. It was predicted that there were 416 recognition sites for C/ EBPa in the 303 lncRNA promoter region, and 13 adipogenesis-promoting miRNAs and five adipogenesis-depressing miRNAs target these lncRNAs. Eighteen lncRNAs, including nine up-and nine down-regulated had more than 2-fold differential expression between the castrated and intact male pigs (q-value < 0.05). Functional analysis indicated that these 18 lncRNAs and their target genes were involved in fatty acid, insulin, and the adipocytokine signaling pathway. We further analyzed the features of a conserved mouse lncRNA gene ENSMUST00000189966 and found it mainly expressed in the cell nucleus and target the Nuclear Receptor Subfamily 2 Group F Member 2 (NR2F2) gene. In 3 T3-L1 cells, differentiation down-regulated their expression, but dihydrotestosterone (DHT) significantly up-regulated their expression in a concentration-dependent manner (P < 0.05). Conclusions: These results suggested that lncRNAs and their target genes might participated in the castration-induced fat deposition and provide a new therapeutic target for combatting testosterone deficiency-related obesity.

  • 相关文献

[1]Long non-coding RNA analysis of muscular responses to testosterone deficiency in Huainan male pigs. Xing, Baosong,Bai, Xianxiao,Guo, Hongxia,Chen, Junfeng,Hua, Liushuai,Zhang, Jiaqing,Ma, Qiang,Ren, Qiaoling,Wang, Jing,Wang, Huashuai.

[2]猪PAANCR组织表达谱分析及其对脂质生成的影响. 王璟,李庆东,滑留帅,陈俊峰,张家庆,任巧玲,刘付玖,张华,曹海,徐照学,邢宝松,白献晓. 2019

[3]Castration-induced changes in the expression profiles and promoter methylation of the GHR gene in Huainan male pigs. Wang, Jing,Chen, Junfeng,Zhang, Jiaqing,Gao, Binwen,Bai, Xianxiao,Lan, Yali,Lin, Ping,Guo, Hongxia,Xing, Baosong,Gao, Yuan.

[4]Cloning and characterization of porcine Fc gamma receptor II (FcR gamma II). Qiao, Songlin,Zhang, Gaiping,Mac, Chun,Zhang, Hong,Zhang, Yuyang,Xi, Jun,Song, Haitao,Li, Xuewu. 2006

[5]Molecular cloning and expression of the porcine high-affinity immunoglobulin G Fc receptor (Fc gamma RI). Zhang, Gaiping,Qiao, Songlin,Li, Qingmei,Wang, Xuannian,Duan, Yanhua,Wang, Li,Xiao, Zhijun,Xia, Chun. 2006

[6]Integrated analysis of non-coding RNA and mRNA expression profiles of 2 pig breeds differing in muscle traits. Sun, J.,Xie, M.,Huang, Z.,Chen, T.,Xi, Qianyun,Wu, T.,Zhang, Y.,Li, H.,Sun, R.,Wang, J.. 2017

[7]Molecular characterization of Japanese encephalitis viruses circulating in pigs and mosquitoes on pig farms in the Chinese province of Henan. Teng, Man,Luo, Jun,Wang, Xing-Tao,Zhang, Gai-Ping,Fan, Jian-Ming,Yao, Wu,Chen, Lu,Wang, Chuan-Qing.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台