文献类型： 外文期刊

作者： Gao, Chun-Qi ¹ ; Xu, Yin-Long ¹ ; Jin, Cheng-Long ¹ ; Hu, Xiao-Chao ¹ ; Li, Hai-Chang; Xing, Guang-Xu; Yan, Hui- ¹ ;

作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Anim Nutr & Regulat, Natl Engn Res Ctr Breeding Swine Ind, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China

2.Guangzhou United Biotechnol Feed Co Ltd, Guangzhou, Guangdong, Peoples R Chi

关键词： satellite cells;differentiation capacity;mTOR signaling pathway

期刊名称：ONCOTARGET （ 影响因子：5.168； 五年影响因子：5.312 ）

ISSN： 1949-2553

年卷期： 2017 年 8 卷 26 期

页码：

收录情况： SCI

摘要： We isolated and cultured satellite cells (SCs) from the longissimus dorsi muscles of 1- day- old male Landrace and Lantang piglets to compare the SC differentiation capacity in the two breeds. Lantang piglets yielded more (P < 0.05) SCs per gram of muscle than Landrace piglets (5.2 +/- 0.9x10(4) vs. 2.4 +/- 0.2x10(4)). Transcription of the differentiation markers myogenin and myosin heavy chain I (MyHC I) in the longissimus dorsi muscle was higher in Lantang than Landrace piglets (P < 0.05). Protein levels of myogenin (P < 0.05), MyHC I (P < 0.05), and myogenic regulatory factor 4 (P = 0.07) were higher in Lantang than Landrace piglet SCs after 72 h of differentiation. Creatine kinase activity was higher in Lantang than Landrace piglet SCs after 24, 48, and 72 h of differentiation (P < 0.05), and there was a greater fusion index in Landrace piglet SCs after 72 h of differentiation. In addition, phosphorylation of Akt, mTOR, S6K1, S6, and 4EBP1 was lower in Lantang than Landrace piglet SCs (P < 0.05). Thus differentiation was more extensive in Lantang than Landrace piglet SCs, but expression of the mTOR signaling pathway was lower in Lantang piglet SCs, suggesting mTOR signaling may inhibit myogenic differentiation. These findings reveal that mTOR signaling is a factor in myogenesis and imply that mTOR could potentially serve as an activator of myoblast differentiation and muscle regeneration.