Main Factors Influencing the Efficient Propagation of Virulent or Attenuated Strains of Japanese encephalitis virus in BHK-21 Cells
文献类型: 外文期刊
作者: Teng, Man 1 ; Luo, Jun 1 ; Xing, Guang-Xu 1 ; Cheng, Na 1 ; Yang, Yan-Yan 1 ; Deng, Rui-Guang 1 ; Zhang, Gai-Ping 1 ;
作者机构: 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
2.Henan Agr Univ, Coll Anim Husb & Vet, Zhengzhou 450002, Peoples R China
关键词: JEV;In vitro culture;TCID50;BHK-21 cell line
期刊名称:INDIAN JOURNAL OF VIROLOGY ( 影响因子:0.364; 五年影响因子:0.287 )
ISSN: 0970-2822
年卷期: 2012 年 23 卷 3 期
页码:
收录情况: SCI
摘要: Japanese encephalitis virus (JEV) causes severe viral encephalitis in humans and some other mammalian animals. Highly efficient culture of the virus is critical for antigen preparation, vaccine production and other basic researches. We have investigated the influence of a number of variables such as the strain virulence, the state of the host cells, medium composition, infection method and others on the proliferation of distinct JEV strains in BHK-21 cells. The results showed that two distinct strategies are needed for the propagation of virulent or attenuated JEV strains. The most critical variables were the method of infection, and especially the density of the host cell. Our studies indicate the general approaches to the in vitro culture of other JEV strains using BHK-21 cell line.
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