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Discovery of human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes and their effects on fertility in male and female mice

文献类型： 外文期刊

作者： Chen, Xuemei 1 ; Liu, Xiaodong 1 ; Ren, Xiuhua 1 ; Li, Xuewu 2 ; Wang, Li 3 ; Zang, Weidong 1 ;

作者机构： 1.Zhengzhou Univ, Basic Med Coll, Dept Human Anat, 100 Kexue Rd, Zhengzhou 450001, Henan, Peoples R China

2.Henan Acad Agr Sci, Minist Agr, Henan Prov Key Lab Anim Immunol, Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

3.Zhengzhou Univ, Basic Med Coll, Dept Pathogen Microorganism, 100 Kexue Rd, Zhengzhou 450001, Henan, Peoples R China

关键词： anti-sperm antibodies (ASAs);gene-specific peptide libraries;immunocontraceptive vaccine;immunoinfertile;male and female mice;phage display;sperm;synthetic peptide

期刊名称：REPRODUCTION FERTILITY AND DEVELOPMENT （ 影响因子：2.311； 五年影响因子：2.396 ）

ISSN： 1031-3613

年卷期： 2016 年 28 卷 4 期

页码：

收录情况： SCI

摘要： The key goals of immunocontraception research are to obtain full contraceptive effects using vaccines administered to both males and females. Current research concerning human anti-sperm contraceptive vaccines is focused on delineating infertility-related epitopes to avoid autoimmune disease. We constructed phage-display peptide libraries to select epitope peptides derived from human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) using sera collected from infertile women harbouring anti-sperm antibodies. Following five rounds of selection, positive colonies were reconfirmed for reactivity with the immunoinfertile sera. We biopanned and analysed the chemical properties of four epitope peptides, named P82, Sa6, Sa37 and Sa76. Synthetic peptides were made and coupled to either bovine serum albumin (BSA) or ovalbumin. We used the BSA-conjugated peptides to immunise BALB/c mice and examined the effects on fertility in female and male mice. The synthetic peptides generated a sperm-specific antibody response in female and male mice that caused a contraceptive state. The immunocontraceptive effect was reversible and, with the disappearance of peptide-specific antibodies, there was complete restoration of fertility. Vaccinations using P82, Sa6 and Sa76 peptides resulted in no apparent side effects. Thus, it is efficient and practical to identify epitope peptide candidates by phage display. These peptides may find clinical application in the specific diagnosis and treatment of male and female infertility and contraceptive vaccine development.

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[1]Analysis of the linear epitope for Fc-binding on the mouse IgG Fc receptor (moFc gamma RI) by synthetic peptide. Wang, F. Y.,Guo, J. Q.,Zhang, G. P.. 2014

[2]Development of a Synthetic Peptide ELISA Assay for the Detection of Foot-and-Mouth Disease Virus Nonstructural Protein Antibodies. Yang Ji-fei,Yang Su-zhen,Yang Yan-yan,Zhi Ai-min,Zhao Dong,Zhi Yu-bao,Xing Guang-xu,Deng Rui-guang,Chai Shu-jun,Zhang Gai-ping,Yang Su-zhen. 2010

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