您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of early salt stress responsive proteins in seedling roots of upland cotton (Gossypium hirsutum L.) employing iTRAQ-based proteomic technique

文献类型： 外文期刊

作者： Li, Wu 1 ; Zhao, Fu'an 2 ; Fang, Weiping 2 ; Xie, Deyi 2 ; Hou, Jianan 2 ; Yang, Xiaojie 2 ; Zhao, Yuanming 2 ; Tang, Zho 1 ;

作者机构： 1.Henan Univ, Coll Life Sci, Kaifeng, Peoples R China

2.Henan Acad Agr Sci, Econ Crop Res Inst, Zhengzhou 450002, Peoples R China

关键词： Gossypium hirsutum;salt stress;iTRAQ;root;proteomics

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2015 年 6 卷

页码：

收录情况： SCI

摘要： Soil salinity is a major abiotic stress that limits plant growth and agricultural productivity. Upland cotton (Gossypium hirsutum L.) is highly tolerant to salinity; however, large-scale proteomic data of cotton in response to salt stress are still scant. Here, an isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomic technique was employed to identify the early differentially expressed proteins (DEPs) from salt-treated cotton roots. One hundred and twenty-eight DEPs were identified, 76 of which displayed increased abundance and 52 decreased under salt stress conditions. The majority of the proteins have functions related to carbohydrate and energy metabolism, transcription, protein metabolism, cell wall and cytoskeleton metabolism, membrane and transport, signal transduction, in addition to stress and defense. It is worth emphasizing that some novel salt-responsive proteins were identified, which are involved in cell cytoskeleton metabolism (actin-related protein2, ARP2, and fasciclin-like arabinogalactan proteins, FLAs), membrane transport (tonoplast intrinsic proteins, TIPs, and plasma membrane intrinsic proteins, PIPs), signal transduction (leucine-rich repeat receptor-like kinase encoding genes, LRR-RLKs) and stress responses (thaumatin-like protein, TLP, universal stress protein, USP, dirigent-like protein, DIR, desiccation-related protein PCC13-62). High positive correlation between the abundance of some altered proteins (superoxide dismutase, SOD, peroxidase, POD, glutathione S-transferase, GST, monodehydroascorbate reductase, MDAR, and malate dehydrogenase, MDH) and their enzyme activity was evaluated. The results demonstrate that the iTRAQ-based proteomic technique is reliable for identifying and quantifying a large number of cotton root proteins. gRT-PCR was used to study the gene expression levels of the five above-mentioned proteins; four patterns are consistent with those of induced protein. These results showed that the proteome of cotton roots under NaCI stress is complex. The comparative protein profiles of roots under salinity vs control improves the understanding of the molecular mechanisms involved in the tolerance of plants to salt stress. This work provides a good basis for further functional elucidation of these DEPs using genetic and/or other approaches, and, consequently, candidate genes for genetic engineering to improve crop salt tolerance.

  • 相关文献

[1]Effects of phosphorus and zinc fertilizer on cadmium uptake and distribution in flax and durum wheat. Jiao, Y,Grant, CA,Bailey, LD. 2004

[2]Inheritance of time of flowering in upland cotton under natural conditions. J. J. HAO,S. X. YU,;Q. X. MA,S. L. FAN,M. Z. SONG.

[3]Effect of temperature on conidial germination, mycelial growth and aggressiveness of the defoliating and nondefoliating pathotypes of Verticillium dahliae from cotton in China. Xu, Fei,Yang, Long,Zhang, Jing,Li, Guoqing,Xu, Fei,Yang, Long,Zhang, Jing,Li, Guoqing,Xu, Fei,Guo, Xiaoping,Zhang, Xianlong. 2012

[4]Comparative Proteomic Analysis of Gossypium thurberi in Response to Verticillium dahliae Inoculation. Weiping Fang,Deyi Xie,Heqin Zhu,Wu Li,Zhenzhen Xu,Lirong Yang,Zhifang Li,Li Sun,Jinxia Wang,Lihong Nie,Zhongjie Tang,Shuping Lv,Fu’an Zhao,Yao Sun,Yuanming Zhao,Jianan Hou,Xiaojie Yang. 2015

[5]Comparative Proteomic Analysis of the Response of Maize (Zea mays L.) Leaves to Long Photoperiod Condition. Wu, Liuji,Tian, Lei,Wang, Shunxi,Liu, Ping,Tian, Zhiqiang,Zhang, Huimin,Chen, Yanhui,Wu, Liuji,Tian, Lei,Wang, Shunxi,Liu, Ping,Tian, Zhiqiang,Zhang, Huimin,Chen, Yanhui,Wu, Liuji,Tian, Lei,Wang, Shunxi,Liu, Ping,Tian, Zhiqiang,Zhang, Huimin,Chen, Yanhui,Zhang, Jun,Liu, Haiping. 2016

[6]iTRAQ-based quantitative proteomics analysis of rice leaves infected by Rice stripe virus reveals several proteins involved in symptom formation. Hajano, Jamal-U-Ddin,Wang, Xifeng,Ren, Yingdang,Lu, Chuantao. 2015

[7]Long photoperiod affects the maize transition from vegetative to reproductive stages: a proteomic comparison between photoperiod-sensitive inbred line and its recurrent parent. Tian, Lei,Wang, Shunxi,Song, Xiaoheng,Liu, Ping,Chen, Zan,Chen, Yanhui,Wu, Liuji,Tian, Lei,Wang, Shunxi,Song, Xiaoheng,Liu, Ping,Chen, Zan,Chen, Yanhui,Wu, Liuji,Tian, Lei,Wang, Shunxi,Song, Xiaoheng,Liu, Ping,Chen, Zan,Chen, Yanhui,Wu, Liuji,Zhang, Jun. 2018

[8]Effect of alpha-lipoic acid on relieving ammonia stress and hepatic proteomic analyses of broilers. Lu, M.,Yin, Q. Q.,Lu, M.,Bai, J.,Xu, B.,Sun, Q. Y.,Wei, F. X.,Li, J.,Wang, G. L.,Li, S. Y.,Tang, X. F.,Zhang, H. F..

[9]Molybdenum Affects Photosynthesis and Ionic Homeostasis of Chinese Cabbage under Salinity Stress. Hu, Chengxiao,Sun, Xuecheng,Zhao, Xiaohu,Tan, Qiling,Zhang, Ying,Zhang, Mu,Li, Na.

[10]Expression profiling and functional characterization of a CBL-interacting protein kinase gene from Populus euphratica. Lv, Fuling,Xia, Xinli,Yin, Weilun,Zhang, Hechen.

[11]Co-overexpressing a Plasma Membrane and a Vacuolar Membrane Sodium/Proton Antiporter Significantly Improves Salt Tolerance in Transgenic Arabidopsis Plants. Pehlivan, Necla,Sun, Li,Jarrett, Philip,Mishra, Neelam,Zhang, Hong,Yang, Xiaojie,Chen, Lin,Shen, Guoxin,Kadioglu, Asim.

[12]Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber. Liu Li-ying,Duan Liu-sheng,Zhang Jia-chang,Zhang Xiao-lan,Zhang Zhen-xian,Ren Hua-zhong,Mi Guo-quan. 2013

[13]The calcium sensor PeCBL1, interacting with PeCIPK24/25 and PeCIPK26, regulates Na+/K+ homeostasis in Populus euphratica. Zhang, Hechen,Lv, Fuling,Han, Xiao,Xia, Xinli,Yin, Weilun,Zhang, Hechen. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台