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Proteomic identification of differentially expressed proteins in Gossypium thurberi inoculated with cotton Verticillium dahliae

文献类型： 外文期刊

作者： Zhao, Fu'an 1 ; Fang, Weiping 1 ; Xie, Deyi 1 ; Zhao, Yuanming 1 ; Tang, Zhongjie 1 ; Li, Wu 1 ; Nie, Lihong 1 ; Lv, Shup 1 ;

作者机构： 1.Henan Acad Agr Sci, Cash Crop Res Inst, Zhengzhou 450002, Peoples R China

2.Henan Univ, Coll Life Sci, Kaifeng 475100, Peoples R China

关键词： Gossypium thurberi;Cotton Verticillium dahliae;Proteome;Two-dimensional electrophoresis;Mass spectrometry;Genetically modified crops

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2012 年 185 卷

页码：

收录情况： SCI

摘要： Thurber's cotton (Gossypium thurberi) is the wild relative of cultivated cotton. It is highly resistant to cotton Verticillium wilt, a disease that significantly affects cotton yield and quality. To reveal the mechanism of disease resistance in G. thurberi and to clone resistance-related genes, we used two-dimensional electrophoresis (2-DE) and tandem time-of-flight mass spectrometry (MALDI-TOF-MS) to identify differentially expressed proteins in Thurber's cotton after inoculation with Verticillium dahliae. A total of 57 different protein spots were upregulated, including 52 known proteins representing 11% of the total protein spots. These proteins are involved in resistance to stress and disease, transcriptional regulation, signal transduction, protein processing and degradation, photosynthesis, production capacity, basic metabolism, and other processes. In addition, five disease resistance proteins showed intense upregulation, indicating that resistance genes (R genes) may play a critical role in resistance to Verticillium wilt in Thurber's cotton. Our results suggest that disease and stress resistance are the combined effects of multiple co-expressed genes. This provides a basis for further, detailed investigation into the mechanisms underlying Verticillium wilt resistance of G. thurberi and for cloning essential genes into cotton cultivars to produce Verticillium wilt resistant plants. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

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