Agrobacterium-mediated transformation of Japanese lawngrass (Zoysia Japonica Steud.) containing a synthetic cryIA(b) gene from Bacillus thuringiensis
文献类型： 外文期刊
作者： Zhang, Lei 1 ; Wu, D. 2 ; Zhang, Li 2 ; Yang, C. 2 ;
作者机构： 1.Henan Acad Agr Sci, Wheat Inst, Zhengzhou 450002, Peoples R China
2.Henan Acad Agr Sci, Wheat Inst, Zhengzhou 450002, Peoples R China; Zhejiang Univ, Inst Nucl Agr Sci, Mutat & Genom Engn Lab, Hangzhou 310029, Peoples R China
关键词： Zoysia japonica;Agrobacterium tumefaciens;genetic manipulation;insecticidal crystal protein
期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）
ISSN： 0179-9541
年卷期： 2007 年 126 卷 4 期
页码：
收录情况： SCI
摘要： Transgenic Japanese lawngrass plants containing a synthetic cryIA (b) gene from Bacillus thuringiensis under the control of a maize ubiquitin promoter were developed by Agrobacterium-mediated transformation. A total of 1540 embryogenic calluses derived from dormancy-removal mature seeds were co-cultured with the disarmed strain EHA105 harbouring the binary vector pKUB. Three days after co-culture with EHA105 in the dark at 21 degrees C, transient beta-glucuronidase (GUS) expression frequency was 74.2%. After selection with 100 mg/l hygromycin B, a total of over 50 independent resistant cell clones and 25 regenerated plants were obtained. The integration and expression of the cryIA(b) gene into the genome was confirmed in 22 regenerated plants by the GUS histochemical assay, PCR amplification, Southern blotting and Western blotting analysis, with a transformation efficiency of 1.4%. The entire process from callus induction of mature seeds to production of transgenic plantlets was 80-100 days. T-1 progeny segregation analysis of these transgenic lines demonstrated that 59.1 % of the transgenic events were inherited in a typical Mendelian fashion.
- 相关文献
作者其他论文 更多>>
-
Analysis of the apoplast fluid proteome during the induction of systemic acquired resistance in Arabidopsis thaliana
作者：Jiang, Shuna;Pan, Liying;Zhou, Qingfeng;Xu, Wenjie;He, Fuge;Gao, Hang;Zhang, Lei
关键词：Proteomics; Differentially accumulated proteins; Systemic acquired resistance; Apoplast; Extracellular space
-
The Complete Genome Sequence Resource of Rhizospheric Soil-Derived Bacillus velezensis Yao, with Biocontrol Potential Against Fusarium solani-Induced Pepper Root Rot
作者：Pei, Dongli;Zhang, Qingchen;Zhu, Xiaoqin;Yao, Xiaolin;Zhang, Lei
关键词：biological control; microbe-genome sequencing
-
Biological Control of Verticillium Wilt and Growth Promotion in Tomato by Rhizospheric Soil-Derived Bacillus amyloliquefaciens Oj-2.16
作者：Pei, Dongli;Zhang, Qingchen;Zhu, Xiaoqin;Zhang, Lei
关键词：Bacillus amyloliquefaciens; Verticillium dahliae; Verticillium wilt; biocontrol; growth promoting
-
Transcript profiling for regulation of sweet potato skin color in Sushu8 and its mutant Zhengshu20
作者：Yang, Yufeng;Wang, Yannan;Zhang, Li;Chen, Xiangong;Yang, Xiaoping;Yang, Guohong;Yang, Yufeng;Xiong, Haizheng;Bhattarai, Gehendra;Ravelombola, Waltram;Olaoye, Dotun;Shi, Ainong;Shi, Dianyi
关键词：Anthocyanin metabolism; Ipomoea batatas; Skin color; WGCNA
-
Genome-wide identification and expression analysis of auxin response factor gene family in Medicago truncatula
作者：Shen, Chenjia;Sun, Tao;Xu, Luqin;Wang, Huizhong;Yang, Yanjun;Yue, Runqing;Tie, Shuanggui;Zhang, Lei
关键词：auxin response factor (ARF);auxin;auxin signaling;nodule formation;Sinorhizobium meliloti infection
-
Genome-wide identification, expression analysis of auxin-responsive GH3 family genes in maize (Zea mays L.) under abiotic stresses
作者：Feng, Shangguo;Yang, Yanjun;Xu, Mingfeng;Wang, Huizhong;Shen, Chenjia;Yue, Runqing;Zhang, Lei
关键词：Abiotic stress;auxin;auxin responsive gene;GH3 gene family;maize
-
Genome-Wide Identification and Expression Profiling Analysis of ZmPIN, ZmPILS, ZmLAX and ZmABCB Auxin Transporter Gene Families in Maize (Zea mays L.) under Various Abiotic Stresses
作者：Yue, Runqing;Tie, Shuanggui;Qi, Jianshuang;Yan, Shufeng;Han, Xiaohua;Sun, Tao;Yang, Yanjun;Wang, Huizhong;Shen, Chenjia;Zhang, Lei
关键词：
