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Characterization of a monoclonal antibody specific for human peripheral myelin protein 22 and its use in immunohistochemical studies of the fetal and adult nervous system

文献类型： 外文期刊

作者： Gregson, Norman A. 1 ; Zhang, Gaiping 2 ; Pritchard, Jane 2 ; Wang, Aiping 3 ; Sanvito, Lara; Hayday, Adrian C.; 1 ;

作者机构： 1.Guys Hosp, Kings Coll London Sch Med, Dept Clin Neurosci, London SE1 1UL, England

2.Guys Hosp, Kings Coll London Sch Med, Dept Clin Neurosci, London SE1 1UL, England; Guys Hosp, Kings Coll London Sch Med, Peter Gorer Dept Immunobiol, London SE1 9RT, England; Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou, Peoples R China; Zhengzhou Univ, Dept Biotechnol, Zhengzhou, Henan Province, Peoples R China

3.Guys Hosp, Kings Coll London Sch Med, Dept Clin Neurosci, London SE1 1UL, England; Guys Hosp, Kings Coll London Sch Med, Peter Gorer Dept Immunobiol, London SE1 9RT, England; Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou, Peoples R China; Zhengzhou Univ, Dep

关键词： immunohistochemistry;inflammatory neuropathy;monoclonal antibody;peripheral myelin protein 22

期刊名称：JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM （ 影响因子：3.494； 五年影响因子：3.455 ）

ISSN： 1085-9489

年卷期： 2007 年 12 卷 1 期

页码：

收录情况： SCI

摘要： We produced a mouse monoclonal antibody using cDNA and peptide immunization against the putative second extra-cellular domain of human peripheral myelin protein 22 (PMP22). It reacted specifically with human PMP22 and not with other human myelin proteins and did not react with bovine, rat, or mouse PMP22. The antibody stained the compact myelin of human peripheral nerve motor and sensory axons and did not stain central nervous system tissue. PMP22 reactivity was detected in the spinal roots of the human fetus at 19-20 weeks of gestation. The staining pattern of the PMP22 antibody resembled that of a monoclonal antibody directed against the myelin protein zero.

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