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Cloning and characterization of porcine Fc gamma receptor II (FcR gamma II)

文献类型: 外文期刊

作者: Qiao, Songlin 1 ; Zhang, Gaiping 2 ; Mac, Chun 2 ; Zhang, Hong 2 ; Zhang, Yuyang 2 ; Xi, Jun 3 ; Song, Haitao; Li, Xue 1 ;

作者机构: 1.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Peoples R China

2.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Peoples R China; Henan Acad Agr Sci, Henan Provincial Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China; China Agr Univ, Coll Vet Med, Beijing 100094, Peoples R China

3.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Peoples R China; Henan

关键词: pig;Fc gamma RII;inhibitory receptor;rosetting

期刊名称:VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY ( 2020影响因子:2.046; 五年影响因子:2.217 )

ISSN: 0165-2427

年卷期: 2006 年 114 卷 1-2 期

页码:

收录情况: SCI

摘要: Receptors for the Fc region (Fc gamma Rs) of IgG play a crucial role in the immune system and host protection against infection. In the present study, we describe the cloning, sequencing and characterization of porcine Fc gamma RII. By screening a translated EST database with the protein sequence of the human Fc gamma RII (CD32) we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), we isolated the full-length cDNA encoding porcine Fc gamma RII from peripheral blood leucocyte RNA. The porcine Fc gamma RII cDNA was 1488 bp long, encoding a 297 amino acid trans-membrane glycoprotein composed of two immunoglobulin-like extracelluar domains, a trans-membrane region and a cytoplasmic tail with an immunoreceptor tyrosine-based inhibitory motif (ITIM). The predicted amino acid sequence was found to be 67% and 52% identical with human and mouse Fc gamma RIIB. RT-PCR indicated porcine Fc gamma RII transcripts expressed in liver, alveolar, mesenteric lymph node and PBLs. COS-7 cells transfected with the pig Fc gamma RII cDNA were able to bind chicken erythrocytes sensitized with porcine IgG. Identification of porcine Fc gamma RII will aid in the understanding IgG-Fc gamma R interactions, and may help in developing new immunization protocols. (c) 2006 Elsevier B.V. All rights reserved.

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