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In vitro expression and monoclonal antibody of RNA-dependent RNA polymerase for infectious bursal disease virus

文献类型： 外文期刊

作者： Zheng, Xiaojuan 1 ; Hong, Lianlian 2 ; Li, Yifei 2 ; Guo, Junqing 2 ; Zhang, Gaiping 3 ; Zhou, Jiyong;

作者机构： 1.Zhejiang Univ, Coll Anim Sci, Inst Prevent Vet Med, Lab Virol & Immunol, Hangzhou 310029, Zhejiang, Peoples R China

2.Zhejiang Univ, Coll Anim Sci, Inst Prevent Vet Med, Lab Virol & Immunol, Hangzhou 310029, Zhejiang, Peoples R China; Zhejiang Univ, Coll Life Sci, Hangzhou 310029, Zhejiang, Peoples R China; Henan Acad Agr Sci, Henan Key Lab Anim Immunol, Zhengzhou, Henan, Peoples R China

3.Zhejiang Univ, Coll Anim Sci, Inst Prevent Vet Med, Lab Virol & Immunol, Hangzhou 310

期刊名称：DNA AND CELL BIOLOGY （ 影响因子：3.311； 五年影响因子：3.557 ）

ISSN： 1044-5498

年卷期： 2006 年 25 卷 11 期

页码：

收录情况： SCI

摘要： VP1, the RNA-dependent RNA polymerase of infectious bursal disease virus (IBDV), has been suggested to play an essential role in the replication and translation of viral RNAs. In this study, we first expressed the complete VP1 protein gene in Escherichia coli (E. coli), and then the produced polyclonal antibody and four monoclonal antibodies (mAbs) to recombinant VP1 protein (rVP1) were shown to bind the IBDV particles in chicken embryo fibroblast and Vero cells. The epitopic analysis showed that mAbs 1D4 and 3C7 recognized respectively two distinct antigenic epitopes on the rVP1 protein, but two pair of mAbs 1A2/2A12 and 1E1/1H3 potentially recognized another two topologically related epitopes. Immunocytochemical stainings showed that VP1 protein formed irregularly shaped particles in the cytoplasm of the IBDV-infected cells. These results demonstrated that the mAbs to rVP1 protein could bind the epitopes of IBDV particles, indicating that the rVP1 protein expressed in E. coli was suitable for producing the mAb to VP1 protein of IBDV, and that the cytoplasm could be the crucial site for viral genome replication of IBDV.

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