Molecular cloning and expression of the porcine high-affinity immunoglobulin G Fc receptor (Fc gamma RI)
文献类型: 外文期刊
作者: Zhang, Gaiping 1 ; Qiao, Songlin 2 ; Li, Qingmei 2 ; Wang, Xuannian 2 ; Duan, Yanhua 3 ; Wang, Li; Xiao, Zhijun; Xi 1 ;
作者机构: 1.Chinese Agr Univ, Coll Vet Med, Beijing 100094, Peoples R China
2.Chinese Agr Univ, Coll Vet Med, Beijing 100094, Peoples R China; Henan Inst Sci & Technol, Coll Anim & Vet Sci, Xinxiang 453003, Peoples R China; Henan Acad Agr Sci, Henan Provincial Key Lab Anim Immunol, Zhenzhou 450002, Peoples R China; Henan Agr Uni, Coll Vet Med, Zhengzhou 450002, Peoples R China
3.Chinese Agr Univ, Coll Vet Med, Beijing 100094, Pe
关键词: cloning;expression;pig;receptor
期刊名称:IMMUNOGENETICS ( 影响因子:2.846; 五年影响因子:2.814 )
ISSN: 0093-7711
年卷期: 2006 年 58 卷 10 期
页码:
收录情况: SCI
摘要: Receptors for the Fc region (Fc gamma Rs) of immunoglobulin G (IgG) play a crucial role in the immune system and host protection against infection. In this study, we describe the cloning, sequencing, and expression of the high-affinity IgG receptor from pig. By screening a translated Expressed Sequence Tags database with the human Fc gamma RI (CD64) protein sequence, we identified a putative porcine homologue. Subsequent polymerase chain reaction amplification confirmed that the identified full-length cDNA was expressed in porcine cells. Rosetting analysis shows that COS-7 cells transfected with a plasmid containing the cloned cDNA were able to bind chicken erythrocytes sensitized with porcine IgG. Scatchard analysis indicated that monomeric IgG bound to transiently transfected cells with an affinity of approximately 4x10(7) M-1. The porcine Fc gamma RI cDNA is 1,038 nucleotides long and is predicted to encode a 346-amino-acid transmembrane glycoprotein composed of three Ig-like domains, a transmembrane region, and a short cytoplasmic tail. The overall identity of the porcine Fc gamma RI to its human and mouse counterparts at the level of the amino acid sequence was 75% and 57%, respectively. Identification of porcine Fc gamma RI will aid in the understanding of the molecular basis of the porcine immune system and further studies of the receptor function.
- 相关文献
作者其他论文 更多>>
-
Potential Pathogenicity and Genetic Characteristics of a Live-Attenuated Classical Swine Fever Virus Vaccine Derivative Variant
作者:Guo, Zhenhua;Xing, Guangxu;Jin, Qianyue;Lu, Qingxia;Zhang, Gaiping;Wang, Leyi;Wang, Leyi;Zhang, Gaiping;Zhang, Gaiping
关键词:
-
An immunochromatographic strip sensor for marbofloxacin residues
作者:Yang, Xingdong;Zhang, Qianqian;Yang, Xingdong;Li, Qingmei;Yang, Jifei;Hu, Xiaofei;Kwee, Sharon
关键词:
-
Proteomic analysis reveals the antiviral effects of baicalin on pseudorabies virus
作者:Niu, Qiaoge;Zhang, Gaiping;Niu, Qiaoge;Zhou, Chuanjie;Li, Rui;Guo, Junqing;Qiao, Songlin;Chen, Xin-xin;Zhang, Gaiping;Zhang, Gaiping
关键词:Baicalin; Pseudorabies virus; ROS; F3; NFU1; CEBPB
-
A rapid simultaneous detection of duck hepatitis A virus 3 and novel duck reovirus based on RPA CRISPR Cas12a/Cas13a
作者:Zhang, Qiaoli;Yu, Guanliu;Zhang, Kaini;Chen, Lei;Ding, Xinli;Sun, Wenbo;Li, Qingmei;Yi, Yunpeng;Wang, Jianhua;Pang, Xuehui
关键词:RPA-CRISPR Cas12a/Cas13a; Duck hepatitis a virus 3; Novel duck reovirus; Lateral flow assay
-
Marine peptides as potential anti-aging agents: Preparation, characterization, mechanisms of action, and future perspectives
作者:Yao, Wanzi;Zhang, Yifeng;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词:Marine peptides; Anti-aging; Preparation; Mechanisms; Oxidative stress; Signaling pathways
-
Thiolated chitosan encapsulation constituted mucoadhesive nanovaccine confers broad protection against divergent influenza A viruses
作者:Ding, Peiyang;Liu, Hongliang;Zhu, Xifang;Chen, Yumei;Zhou, Jingming;Wang, Aiping;Zhang, Gaiping;Ding, Peiyang;Liu, Hongliang;Zhu, Xifang;Chen, Yumei;Zhou, Jingming;Wang, Aiping;Zhang, Gaiping;Ding, Peiyang;Liu, Hongliang;Zhu, Xifang;Chen, Yumei;Zhou, Jingming;Wang, Aiping;Zhang, Gaiping;Chai, Shujun;Zhang, Gaiping;Zhang, Gaiping
关键词:Mucoadhesive; Thiolated chitosan; Influenza nanovaccine; M2e; Nucleoprotein
-
Identification of the Linear Fc-Binding Site on the Bovine IgG1 Fc Receptor (boFcγRIII) Using Synthetic Peptides
作者:Wang, Ruining;Guo, Junqing;Yang, Jifei;Li, Qingmei;Zhang, Gaiping;Wang, Ruining;Li, Ge;Wang, Xun;Zhang, Gaiping;Zhang, Gaiping
关键词:BoFc gamma RIII; Fc-binding site; bovine IgG1; synthetic peptides