您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of two transcription factors activating the expression of OsXIP in rice defence response

文献类型： 外文期刊

作者： Zhan, Yihua 1 ; Sun, Xiangyu 1 ; Rong, Guozeng 2 ; Hou, Chunxiao 3 ; Huang, Yingying 1 ; Jiang, Dean 1 ; Weng, Xiaoyan 1 ;

作者机构： 1.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

2.Cixi Agr Technol Promot Ctr, Cixi 315300, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Rural Dev, Hangzhou 310021, Zhejiang, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Informat, Hangzhou 310021, Zhejiang, Peoples R China

关键词： 5 ' deletion;OsbHLH59;OsERF71;Plant defence;Rice xylanase inhibitor;Transcriptional regulation

期刊名称：BMC BIOTECHNOLOGY （ 影响因子：2.563； 五年影响因子：3.292 ）

ISSN： 1472-6750

年卷期： 2017 年 17 卷

页码：

收录情况： SCI

摘要： Background: Xylanase inhibitors have been confirmed to be involved in plant defence. OsXIP is a XIP-type rice xylanase inhibitor, yet its transcriptional regulation remains unknown. Results: Herbivore infestation, wounding and methyl jasmonate (MeJA) treatment enhanced mRNA levels and protein levels of OsXIP. By analyzing different 5 ' deletion mutants of OsXIP promoter exposed to rice brown planthopper Nilaparvata lugens stress, a 562 bp region (-1451 - -889) was finally identified as the key sequence for the herbivores stress response. Using yeast one-hybrid screening, coupled with chromatin immunoprecipitation analysis, a basic helix-loop-helix protein (OsbHLH59) and an APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor OsERF71 directly binding to the 562 bp key sequence to activate the expression of OsXIP were identified, which is further supported by transient expression assay. Moreover, transcriptional analysis revealed that mechanical wounding and treatment with MeJA resulted in an obvious increase in transcript levels of OsbHLH59 and OsERF71 in root and shoot tissues. Conclusions: Our data shows that two proteins as direct transcriptional activators of OsXIP responding to stress were identified. These results reveal a coordinated regulatory mechanism of OsXIP, which may probably be involved in defence responses via a JA-mediated signaling pathway.

  • 相关文献

[1]Expression regulation of a xylanase inhibitor gene riceXIP in rice (Oryza sativa L.). Zhan, Yihua,Sun, Xiangyu,Xu, Ying,Huang, Yingying,Jiang, Dean,Weng, Xiaoyan,Sun, Renjie,Hou, Chunxiao,Hou, Chunxiao. 2017

[2]Papaya CpEIN3a and CpNAC2 Co-operatively Regulate Carotenoid Biosynthesis-Related Genes CpPDS2/4, CpLCY-e and CpCHY-b During Fruit Ripening. Fu, Chang-chun,Kuang, Jian-fei,Chen, Jian-ye,Lu, Wang-jin,Han, Yan-chao. 2017

[3]Cloning and characterization of the mouse JDP2 gene promoter reveal negative regulation by p53. Xu, Yuanhong,Liu, Zhe,Zhang, Zhongbo,Bi, Shulong,Jin, Chunyuan,Li, Hongjie,Pan, Jianzhi,Yokoyama, Kazunari K..

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台