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Characterization and Comparative Expression Profiling of Browning Response in Medinilla formosana after Cutting

文献类型： 外文期刊

作者： Wang, Yan 1 ; Wang, Yiting 1 ; Liz, Kunfeng 2 ; Song, Xijiao 1 ; Chen, Jianping 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Minist Agr, Key Lab Biotechnol Plant Protect,Inst Virol & Bio, Hangzhou, Zhejiang, Peoples R China

2.Zhejiang Univ, Agr Expt Stn, Hangzhou, Zhejiang, Peoples R China

关键词： transcriptome;high-throughput sequencing;browning;tissue culture;Medinilla;cutting;gene expression profiling

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Plant browning is a recalcitrant problem for in vitro culture and often leads to poor growth of explants and even failure of tissue culture. However, the molecular mechanisms underlying browning-induced physiological processes remain unclear. Medinilla is considered one of the most difficult genera for tissue culture owning to its severe browning. In the present study, intact aseptic plantlets of Meelinilla formosana Hayata previously obtained by ovary culture, were used to explore the characteristics and molecular mechanism of the browning response. Successive morphological and anatomical observations after cutting showed that the browning of M. formosana was not lethal but adaptive. De novo transcriptome and digital gene expression (DGE) profiling using Illumina high-throughput sequencing were then used to explore molecular regulation after cutting. About 7.5 million tags of de novo transcriptome were obtained and 58,073 unigenes were assembled and annotated. A total of 6,431 differentially expressed genes (DEGs) at three stages after cutting were identified, and the expression patterns of these browning-related genes were clustered and analyzed. A number of putative DEGs involved in signal transduction and secondary metabolism were particularly studied and the potential roles of these cutting-responsive mRNAs in plant defense to diverse abiotic stresses are discussed. The DGE profiling data were also validated by quantitative RT-PCR analysis. The data obtained in this study provide an excellent resource for unraveling the molecular mechanisms of browning processes during in vitro tissue culture, and lay a foundation for future studies to inhibit and eliminate browning damage.

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