Genome-Wide Identification of New Reference Genes for qRT-PCR Normalization under High Temperature Stress in Rice Endosperm
文献类型： 外文期刊
作者： Xu, Heng 1 ; Bao, Jian-Dong 2 ; Dai, Ji-Song 1 ; Li, Yongqing 3 ; Zhu, Ying 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China
2.Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Taiwan Joint Ctr Ecol Control Crop Pests, Fuzhou 350002, Peoples R China
3.Chinese Acad Sci, Key Lab South China Agr Plant Mol Anal & Genet Im, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
期刊名称：PLoS One （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2015 年 10 卷 11 期
页码：
收录情况： SCI
摘要： qRT-PCR is one of the most popular approaches to analyze specific gene expression level, and stably expressed reference genes are essential to obtain reliable results. However, many reference genes are only stable under certain circumstances and different reference genes might be required in different experiments. High temperature is a common stress that affects rice endosperm development and it has become a hot topic recently. Although study about reference genes at different developmental stages in rice has been reported, these genes may not be suitable to study high temperature mediated responses especially in endosperm. In our quest for proper reference genes to quantify gene expression in rice endosperm under high temperature, we studied 6 candidate genes selected from the transcriptome data and 11 housekeeping genes. All genes were analyzed with qRT-PCR and the expression stability was assessed with software geNorm and NormFinder. Fb15 and eIF-4a were identified as the two most stable genes in endosperm at different developmental stages, while high temperature treatment has a least effect on expression of Fb15 and UBQ5 in rice endosperm. Our results provide some good candidate reference genes for qRT-PCR normalization in rice endosperm under different temperatures.
- 相关文献
作者其他论文 更多>>
-
Genome-wide identification of HIPP genes family in sorghum reveals the novel role of SbHIPP40 in accumulation of cadmium
作者：Zhang, Heng;Liu, Ziwen;Song, Tao;Han, Yu;Wang, Yao;Shao, Yu;Wang, Fulin;Zou, Guihua;Zhu, Ying;Zhai, Guowei;Liu, Ziwen;Zhu, Zhengge;Ni, Xianlin;Hu, Xiangyang
关键词：Sorghum bicolor; SbHIPP40; HMA domain; Cd accumulation
-
A J-Domain Protein J3 Antagonizes ABI5-BINDING PROTEIN2 to Regulate CONSTANS Stability and Flowering Time
作者：Zhi, Lulu;Mao, Yan;Liang, Lei;Ni, Huanhuan;Li, Ping;Hu, Xiangyang;Zhang, Heng;Zhu, Ying;Huang, Jinling;Huang, Jinling
关键词：AFP2; Arabidopsis; CONSTANS, circadian, growth; J3 protein
-
The Wx/SSIIa and GS3/GW7 Alleles, Both Individually and in Combination, Can Significantly Distinguish Rice Germplasm Quality
作者：Hao, Yuanyuan;Wu, Mingming;Song, Jiayu;Han, Yifei;Li, Chunshou;Huang, Fudeng;Xu, Junfeng;Xu, Junfeng;Zhu, Ying
关键词：rice quality; Wx; SSIIa; GS3; GW7; allelic combination
-
A telomere-to-telomere genome assembly of Chinese grain sorghum 654
作者：Wang, Fulin;Zhang, Heng;Song, Tao;Liu, Zhijian;Han, Yu;Zou, Guihua;Zhu, Ying;Bao, Jiandong;Zhai, Guowei;Yu, Fan
关键词：
-
Genetic structure, dispersal pathways, and northern expansion predictions of Solenopsis invicta: A two-decade journey through China's diverse landscapes
作者：Huang, Jun;Bao, Jian-Dong;Dong, Wan-Ying;Chen, Li-Ming;Ullah, Farman;Zhou, Shu-Xing;Li, Xiao-Wei;Zhang, Jin-Ming;Zhang, Zhi-Jun;Lu, Yao-Bin;Yu, Chen-Di;Wang, Shi-Zhen;Zhang, Juan;Jiang, Zhi-Tao;Su, Hong-Li;Lu, Yao-Bin
关键词：biological invasion; genetic structure; genetic variation; local diffusion distance; red imported fire ant;
Solenopsis invicta -
An Improved Agrobacterium-Mediated Transformation Method for an Important Fresh Fruit: Kiwifruit (Actinidia deliciosa)
作者：Piao, Chun-Lan;Ding, Mengdou;Gao, Yongbin;Cui, Min-Long;Ding, Mengdou;Song, Tao;Zhu, Ying
关键词：kiwifruit (
Actinidia deliciosa ); leaf explant; high-efficiency genetic transformation; Southern blot analysis; mature plant; stableGFP expression -
Phenomics-assisted genetic dissection and molecular design of drought resistance in rice
作者：Lou, Qiaojun;Wang, Xin;Yan, Ming;Feng, Fangjun;Xu, Kai;Gao, Huan;Xia, Hui;Wang, Lei;Li, Tiemei;Chen, Shoujun;Mei, Hanwei;Chen, Liang;Luo, Lijun;Chen, Yunyu;Wang, Xin;Zhang, Yulu;Gao, Tingting;Shi, Jiawei;Xie, Shangyuan;Xi, Xiaoyan;Li, Weikun;Zhang, Jianwei;Yang, Wanneng;Lou, Qiaojun;Zhu, Ying;Lin, Feng;Lou, Qiaojun;Yan, Ming;Feng, Fangjun;Xu, Kai;Gao, Huan;Xia, Hui;Wang, Lei;Li, Tiemei;Chen, Shoujun;Mei, Hanwei;Chen, Liang;Luo, Lijun;Nie, Yuanyuan
关键词：drought resistance; de novo drought-resistant rice genomes; high-throughput phenomics; whole-plant image traits; multimodal camera; quantitative trait loci; QTLs; molecular design
