Antifungal Efficacy of Phellodendron amurense Ethanol Extract against Trichophyton mentagrophytes in Rabbits
文献类型： 外文期刊
作者： Xiao, C. W. 1 ; Ji, Q. A. 1 ; Rajput, Z. I. 2 ; Wei, Q. 1 ; Liu, Y. 1 ; Bao, G. L. 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China
2.Directorate Anim Husb Sindh, Res & Dev Sect, Hyderabad 71000, Pakistan
关键词： Alkaloids;Antifungal;Medicinal plants;Phellodendron chinense;Trichophyton;mentagrophytes
期刊名称：PAKISTAN VETERINARY JOURNAL （ 影响因子：1.318； 五年影响因子：0.954 ）
ISSN： 0253-8318
年卷期： 2014 年 34 卷 2 期
页码：
收录情况： SCI
摘要： The bark of the Phellodendron tree has been used in traditional Chinese medicine for thousands of years. Phellodendron amurense is widely used to treat gastroenteritis, abdominal pain, diarrhea and various inflammatory diseases including arthritis and dermatophytosis. The present study was undertaken to evaluate the antifungal effects of Phellodendron amurense ethanol extract (PAEE) against Trichophyton mentagrophytes in vivo and in vitro. Quantitative analysis revealed that the level of total alkaloids in PAEE was 7.58 +/- 0.46 mg/mL. In the in vitro study, the minimal inhibitory concentration (MIC) of PAEE (0.03g/mL) and clotrimazole (0.02 mg/mL) were determined using 1.5% tryptic soya agar. The influence of different doses of PAEE on the growth of T. mentagrophytes was detected by dry weight determination. Moreover, transmission electronic microscopy was performed to observe the effect of PAEE on cell ultrastructure, and it showed that PAEE destroyed the cell membrane of T. mentagrophytes. Furthermore, the dermatophytosis infection model in rabbits with T. mentagrophytes was established for investigating the in vivo effect of PAEE at 10, 50 and 100% concentrations. The findings in all treatment groups demonstrated the inhibitory effects of PAEE against T. mentagrophytes. Therefore, we conclude that PAEE has significant antifungal activity and could be used to treat T. mentagrophytes infections in rabbits. (C) 2013 PVJ. All rights reserved
- 相关文献
作者其他论文 更多>>
-
Equine chorionic gonadotropin pretreatment 15 days before fixed-time artificial insemination improves the reproductive performance of replacement gilts
作者：Zhao, Q.;Tao, C.;Wei, Q.;Wang, L.;Liu, M.;Li, J.;Pan, J.;Zhu, Z.;Huang, J.;Yu, F.;Chen, X.;Zhang, L.
关键词：Follicular development; Ovulation; Pigs; Reproductive technique; Total pigs born
-
Short communication. Enhancement of the immune responses to vaccination against foot-and-mouth disease in mice by oral administration of Quillaja saponaria-A and extracts of Cochinchina momordica seed
作者：Xiao, C. W.;Bao, G.;Rajput, Z. I.;Hu, S. H.;Rajput, Z. I.;Soomro, N. A.
关键词：adjuvant;ELISA;oral immunization;vaccine
-
Cloning and RNAi-mediated functional characterization of MaLac2 of the pine sawyer, Monochamus alternatus
作者：Niu, B. -L.;Shen, W. -F.;Liu, Y.;Weng, H. -B.;He, L. -H.;Meng, Z. -Q.;Tao, Y. -Z.;Mu, J. -J.;Wu, Z. -L.;Jiang, P.
关键词：Monochamus alternatus;cuticle sclerotization;MaLac2;RNA interference (RNAi);laccase 2
-
EFFECT OF A LYTIC BACTERIOPHAGE ON RABBITS EXPERIMENTALLY INFECTED WITH PATHOGENIC ESCHERICHIA COLI
作者：Zhao, J.;Yao, H.;Bao, G.;Zhao, J.;Pan, L.;Liu, Y.;Bao, G.;He, L.
关键词：atypical enteropathogenic Escherichia coil;bacteriophage;phage therapy;rabbit
-
Genome wide linkage disequilibrium in Chinese asparagus bean (Vigna. unguiculata ssp. sesquipedialis) germplasm: Implications for domestication history and genome wide association studies
作者：Xu, P.;Wu, X.;Wang, B.;Liu, Y.;Lu, Z.;Wang, S.;Li, G.;Luo, J.;Ehlers, J. D.;Close, T. J.;Roberts, P. A.
关键词：asparagus bean;association mapping;cowpea;domestication history;linkage disequilibrium;population structure
-
Enhancement of immune responses to infectious bursal disease vaccine by supplement of an extract made from Momordica cochinchinensis (Lour.) Spreng. seeds
作者：Rajput, Z. I.;Xiao, C. W.;Hu, S. H.;Rajput, Z. I.;Xiao, C. W.;Habib, M.;Soomro, N. A.
关键词：immune response;growth performance;mitogenic stimulated lymphocyte proliferation
