您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Establishment of a highly efficient regeneration method from the scraped broken embryo of mature barley seed

文献类型： 外文期刊

作者： Hua, Wei 1 ; Zhu, Jinghuan 1 ; Shang, Yi 1 ; Wang, Junmei 1 ; Jia, Qiaojun 1 ; Lin, Feng 1 ; Yang, Jianming 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Natl Barley Improvement Ctr, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Barley;callus induction;mature embryo;plant regeneration;scraped broken embryo

期刊名称：CANADIAN JOURNAL OF PLANT SCIENCE （ 影响因子：1.018； 五年影响因子：1.242 ）

ISSN： 0008-4220

年卷期： 2013 年 93 卷 6 期

页码：

收录情况： SCI

摘要： An efficient and reproducible plant regeneration system for mature barley would represent a significant innovation for barley tissue culture and genetic transformations to optimize barley crop traits. To address this need, we used scraped broken embryos (SBEs) from dried barley seeds to establish a highly efficient regeneration system. Mature SBEs cultured in an induction medium with 2 mg L-1 2,4-D offered the best callus formation (92.99% induction efficiency) and no germination. Furthermore, call arising from SBEs had the highest regeneration frequency (69.72%) in FHG medium with 1 mg L-1 6-benzylaminopurine. We then compared three different cultivars: Golden Promise, Zaoshu3, and Zhepi8 for callogenesis and regeneration using an established protocol. Zhepi8 had the greatest callus induction (92.78%) and regeneration (68.24%). Our innovative methods for mature barley plant reproduction will contribute to improvements in genetic barley transformation as an alternate regeneration system.

  • 相关文献

[1]Shoot organogenesis and plant regeneration in Agave hybrid, No. 11648. Zhang, Yan-Mei,Li, Jun-Feng,Lu, Jun-Ying,Zhou, Wen-Zhao,Li, Xin,Chen, Zhi. 2013

[2]Protoplast isolation and plant regeneration of different doubled haploid lines of cauliflower (Brassica oleracea var. botrytis). Sheng, Xiaoguang,Zhao, Zhenqing,Yu, Huifang,Wang, Jiansheng,Xiaohui, Zhang,Gu, Honghui. 2011

[3]Pathotypes and Genetic Diversity of Blumeria graminis f sp hordei in the Winter Barley Regions in China. Zhu Jing-huan,Wang Jun-mei,Jia Qiao-jun,Yang Jian-ming,Lin Feng,Hua Wei,Shang Yi,Zhu Jing-huan,Zhou Yi-jun,Zhou Yi-jun. 2010

[4]Toward Identification of Black Lemma and Pericarp Gene Blp1 in Barley Combining Bulked Segregant Analysis and Specific-Locus Amplified Fragment Sequencing. Jia, Qiaojun,Liang, Zongsuo,Jia, Qiaojun,Liang, Zongsuo,Wang, Junmei,Zhu, Jinghuan,Hua, Wei,Shang, Yi,Yang, Jianming. 2017

[5]Virulence and diversity of Blumeria graminis f.sp hordei in East China. Dreiseitl, Antonin,Wang, Junmei. 2007

[6]Marker development using SLAF-seq and whole-genome shotgun strategy to fine-map the semi-dwarf gene ari-e in barley. Jia, Qiaojun,Jia, Qiaojun,Tan, Cong,Zhang, Xiao-Qi,Luo, Hao,Li, Chengdao,Wang, Junmei,Zhu, Jinghuan,Yang, Jianming,Westcott, Sharon,Broughton, Sue,Moody, David. 2016

[7]Molecular characterization and functional analysis of barley semi-dwarf mutant Riso no. 9265. Jia, Qiaojun,Zhang, Guoping,Jia, Qiaojun,Shang, Yi,Zhu, Jinghuan,Hua, Wei,Wang, Junmei,Yang, Jianming,Li, Chengdao. 2015

[8]Identification of Suitable Reference Genes for Barley Gene Expression Under Abiotic Stresses and Hormonal Treatments. Hua, Wei,Zhu, Jinghuan,Shang, Yi,Wang, Junmei,Jia, Qiaojun,Yang, Jianming.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台