文献类型： 外文期刊
作者： Zhang, Yan-Mei 1 ; Li, Xin 2 ; Chen, Zhi 3 ; Li, Jun-Feng 1 ; Lu, Jun-Ying 1 ; Zhou, Wen-Zhao 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Hainan Prov Key Lab Trop Crops Nutr, Zhanjiang 524091, Peoples R China
2.Hubei Inst Nationalities, Sch Biol Sci & Technol, Enshi 445000, Peoples R China
3.Zhejiang Acad Agr Sci, Virol & Biotechnol Res Inst, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Agave hybrid No. 11648;Callus induction;Shoot organogenesis;Plant regeneration
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）
ISSN： 0304-4238
年卷期： 2013 年 161 卷
页码：
收录情况： SCI
摘要： The effects of explant type and the combination and concentration of plant growth regulators on callus induction and shoot regeneration in Agave hybrid No. 11648 were examined. Shoot tip and immature leaf segments were cultured on Murashige and Skoog (MS) medium supplied with different concentrations of benzyladenine (BA), at 0.2-3.0 mg l(-1), and alpha-naphthalene acetic acid (NAA), at 0.1-1.0 mg l(-1), or 2,4-dichlorophenoxyacetic acid (2,4-D), at 0.2-2.0 mg l(-1) for callus induction. The highest rate of light yellowgreen, compact and nodular-like calli was formed on the MS medium supplemented with 2.0 mg l(-1) BA in combination with 0.2 mg l(-1) NAA, with 93.2% of shoot tips producing a compact, light yellowgreen and nodular-like callus. The calli derived from the different explants were subcultured on Schenk and Hildebrandt (SH) medium (300 mg l(-1) ammonium nitrate, 2267 mg l(-1) potassium nitrate, 353 mg l(-1) potassium dihydrogen phosphate, 3 mg l(-1) glycine, 1.0 mg l(-1) thiamine hydrochloride (B-1), 1.0 mg l(-1) pyridoxine hydrochloride (B6), 1.0 mg l(-1) niacin, 200 mg l(-1) idositol) containing different concentrations of BA, NAA and indole-3-butyric acid (IBA) for shoot induction. Shoot organogenesis was achieved when the callus tissue derived from shoot tips was transferred to SH medium. Maximum organogenesis was obtained with 5.0 mg l(-1) BA in combination with 0.1 mg l(-1) NM and 0.1 mg l(-1) IBA, yielding 98.0% shoot induction frequencies. Regenerated shoots were rooted on growth regulator-free MS medium, with an average number of 5.39 roots per shoot, and a length of 8.44 cm per root within'30 days, and acclimatized in a greenhouse, with 98.57 1.43% survival. This is the first report of plant regeneration from Agave hybrid No. 11648 shoot tips. (C) 2013 Elsevier B.V. All rights reserved.
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