Evaluation of Bacterial Expansin EXLX1 as a Cellulase Synergist for the Saccharification of Lignocellulosic Agro-Industrial Wastes
文献类型： 外文期刊
作者： Lin, Hui 1 ; Shen, Qi 1 ; Zhan, Ju-Mei 2 ; Wang, Qun 1 ; Zhao, Yu-Hua 1 ;
作者机构： 1.Zhejiang Univ, Coll Life Sci, Inst Microbiol, Hangzhou 310003, Zhejiang, Peoples R China
2.Zhejiang Univ, Coll Life Sci, Inst Plant Sci, Hangzhou 310003, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Environm Resource Soil & Fertilizer, Hangzhou, Zhejiang, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2013 年 8 卷 9 期
页码：
收录情况： SCI
摘要： Various types of lignocellulosic wastes extensively used in biofuel production were provided to assess the potential of EXLX1 as a cellulase synergist. Enzymatic hydrolysis of natural wheat straw showed that all the treatments using mixtures of cellulase and an optimized amount of EXLX1, released greater quantities of sugars than those using cellulase alone, regardless of cellulase dosage and incubation time. EXLX1 exhibited different synergism and binding characteristics for different wastes, but this can be related to their lignocellulosic components. The cellulose proportion could be one of the important factors. However, when the cellulose proportion of different biomass samples exhibited no remarkable differences, a higher synergism of EXLX1 is prone to occur on these materials, with a high proportion of hemicellulose and a low proportion of lignin. The information could be favorable to assess whether EXLX1 is effective as a cellulase synergist for the hydrolysis of the used materials. Binding assay experiments further suggested that EXLX1 bound preferentially to alkali pretreated materials, as opposed to acid pretreated materials under the assay condition and the binding preference would be affected by incubation temperature.
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