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Oxidative injury is involved in fipronil-induced G2/M phase arrest and apoptosis in Spodoptera frugiperda (Sf9) cell line

文献类型： 外文期刊

作者： Wang, Xin-quan 1 ; Li, You-gui 2 ; Zhong, Shi 3 ; Zhang, Hu 1 ; Wang, Xiang-yun 1 ; Qi, Pei-pei 1 ; Xu, Hao 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, State Key Lab Breeding Base Zhejiang Sustainable, Key Lab Detect Pesticide Residues,Minist Agr, Hangzhou, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Zhejiang, Peoples R China

3.Zhejiang Acad

关键词： Fipronil;Sf9 cells;Oxidative injury;G2/M phase arrest;Apoptosis

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2013 年 105 卷 2 期

页码：

收录情况： SCI

摘要： Fipronil (FPN) is a phenylpyrazole insecticide acted on insect gamma aminobutyric acid (GABA) receptors. Although action of FPN is restricted on insect neuronal or muscular transmitter system, a few studies have assessed the toxicity and its underlying mechanisms on the insect reproduce. To determine whether FPN-induced reproductive hazards on insect, we used the insect cells (Sf9) derived from Spodoptera frugiperda pupal ovarian as an in vitro model. FPN was cytotoxic to Sf9 cells with a time-and concentration-dependent manner. After Sf9 cells exposure to FPN (400 mu g/mL) for 48 h, flow cytometry analysis showed FPN-induced Sf9 cells G2/M phase arrest, the percentage of G2/M phase cells evidently increased from 57.08 +/- 0.29% to 90.48 +/- 1.75%, whilst, the proportion of apoptotic cells are also significantly increased from 4.48 +/- 0.52% to 19.75 +/- 1.01%. Morphological observation found FPN treated cells showed typical apoptosis morphological changes including cell swelling, apoptotic bodies and small fragments. Western blotting revealed Cdk1, cyclin A, cyclin B1 and BcI-2 were markedly down-regulated, and cytosolic Cyt c, caspase-3 expressions were significantly augmented in FPN-treated Sf9 cells. Intracellular biochemical assays testified that FPN-induced G2/M cell cycle arrest and apoptosis involved in increase of ROS generation, especially (OH)-O-center dot and depleted GSH level and Na+-K+-ATPase, Ca2+-Mg2+-ATPase activities. The results provide a new point of view to study the toxicology and mode of action of insecticide on cellular level. (C) 2013 Elsevier Inc. All rights reserved.

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